[NMR paper] NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation
NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation
Many RNA-binding proteins undergo liquid-liquid phase separation, which underlies the formation of membraneless organelles, such as stress granules and P-bodies. Studies of the molecular mechanism of phase separation in vitro are hampered by the coalescence and sedimentation of organelle-sized droplets interacting with glass surfaces. Here, we demonstrate that liquid droplets of fused in sarcoma (FUS)-a protein found in cytoplasmic aggregates of amyotrophic lateral sclerosis and frontotemporal...
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[NMR paper] DiffErential EPitope mapping by STD NMR spectroscopy (DEEP-STD NMR) to reveal the nature of protein-ligand contacts.
DiffErential EPitope mapping by STD NMR spectroscopy (DEEP-STD NMR) to reveal the nature of protein-ligand contacts.
DiffErential EPitope mapping by STD NMR spectroscopy (DEEP-STD NMR) to reveal the nature of protein-ligand contacts.
Angew Chem Int Ed Engl. 2017 Oct 04;:
Authors: Monaco S, Tailford LE, Juge N, Angulo J
Abstract
STD NMR spectroscopy is extensively used to obtain epitope maps of ligands binding to protein receptors, revealing structural details of the interaction, which is key to direct lead optimization efforts...
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10-05-2017 07:28 PM
[NMR paper] DiffErential EPitope mapping by STD NMR spectroscopy (DEEP-STD NMR) to reveal the nature of protein-ligand contacts
DiffErential EPitope mapping by STD NMR spectroscopy (DEEP-STD NMR) to reveal the nature of protein-ligand contacts
STD NMR spectroscopy is extensively used to obtain epitope maps of ligands binding to protein receptors, revealing structural details of the interaction, which is key to direct lead optimization efforts in drug discovery. However, it does not give information about the nature of the amino acids surrounding the ligand in the binding pocket. Here we report the development of a novel protocol, Differential Epitope Mapping by STD NMR (DEEP-STD NMR), to identify the type of...
NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation - Nature.com
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