New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Digital Journal

		BioNMR > NMR community > Online News
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Digital Journal

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-20-2016, 04:54 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Digital Journal

New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Digital Journal

New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm
Digital Journal
Recently, labeling of proteins and peptides with stable heavy isotopes (deuterium, carbon-13, nitrogen-15, and oxygen-18) has been widely used in quantitative proteomics. In fact, such labeling has long been a staple of NMR spectroscopy studies. It can ...

and more »
Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Press Release Rocket New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Press Release Rocket <img alt="" height="1" width="1"> New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm Press Release Rocket Recently, labeling of proteins and peptides with stable heavy isotopes (deuterium, carbon-13, nitrogen-15, and oxygen-18) has been widely used in quantitative proteomics. In fact, such labeling has long been a staple of NMR spectroscopy studies. It can ... and more » Read here	nmrlearner	Online News	0	04-19-2016 04:52 AM
Protein Electrophoresis Market - Global Industry Analysis, Market Trends and ... - Digital Journal <img alt="" height="1" width="1"> Protein Electrophoresis Market - Global Industry Analysis, Market Trends and ... Digital Journal ... are most common and inexpensive procedures, they are followed by the highly accurate quantitative preparative native continuous PAGE (QPNC-PAGE) that employs Nuclear Magnetic Resonance spectrometer to study molecules of protein polypeptide. Protein Electrophoresis Market - Global Industry Analysis, Market Trends and ... - Digital Journal More...	nmrlearner	Online News	0	12-08-2015 08:28 PM
Stable isotope labeling strategy based on coding theory Stable isotope labeling strategy based on coding theory Abstract We describe a strategy for stable isotope-aided protein nuclear magnetic resonance (NMR) analysis, called stable isotope encoding. The basic idea of this strategy is that amino-acid selective labeling can be considered as â??encoding and decodingâ?? processes, in which the information of amino acid type is encoded by the stable isotope labeling ratio of the corresponding residue and it is decoded by analyzing NMR spectra. According to the idea, the strategy can diminish the...	nmrlearner	Journal club	0	08-21-2015 09:52 AM
Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule Abstract Silkworms serve as promising bioreactors for the production of recombinant proteins, including glycoproteins and membrane proteins, for structural and functional protein analyses. However, lack of methodology for stable isotope labeling has been a major deterrent to using this expression system for nuclear magnetic resonance (NMR) structural biology. Here we developed a metabolic isotope labeling technique using commercially...	nmrlearner	Journal club	0	04-23-2015 04:37 AM
Global Protein Crystallization Market - Industry Segments, Trends Analysis and ... - Digital Journal Global Protein Crystallization Market - Industry Segments, Trends Analysis and ... - Digital Journal <img alt="" height="1" width="1"> Global Protein Crystallization Market - Industry Segments, Trends Analysis and ... Digital Journal Various techniques used during protein crystallization technology are ion-exchange chromatography, high-performance liquid chromatography (HPLC), gel-electrophoresis, x-ray crystallography and nuclear magnetic resonance (NMR). On the basis of ... Read here	nmrlearner	Online News	0	04-02-2015 10:26 PM
Global Protein Crystallization Market - Size Share, Segment Analysis and ... - Digital Journal Global Protein Crystallization Market - Size Share, Segment Analysis and ... - Digital Journal <img alt="" height="1" width="1"> Global Protein Crystallization Market - Size Share, Segment Analysis and ... Digital Journal Various techniques used during protein crystallization technology are ion-exchange chromatography, high-performance liquid chromatography (HPLC), gel-electrophoresis, x-ray crystallography and nuclear magnetic resonance (NMR). On the basis of ... Read here	nmrlearner	Online News	0	02-27-2015 11:25 PM
Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products http://www.spectroscopynow.com/common/images/thumbnails/13abcca009b.jpgA quantitative NMR method has been reported for quantitative analysis of three medicinal plant extracts and their herbal products without the need of authentic standards. Source: Spectroscopynow.com	nmrlearner	General	0	02-03-2013 08:49 AM
[NMR paper] Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis. Related Articles Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis. J Biomol NMR. 1995 Sep;6(2):129-34 Authors: Kigawa T, Muto Y, Yokoyama S For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity...	nmrlearner	Journal club	0	08-22-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off