Nanodiscs catch misfolding proteins Xinhua
"We're able to stop the aggregation of the protein in this restricted membrane environment so we can monitor what it looks like before it becomes a mass of fibers," said lead researcher Ayyalusamy Ramamoorthy, UM professor of biophysics and chemistry ...
[NMR paper] Insights into protein misfolding and aggregation enabled by solid-state NMR spectroscopy.
Insights into protein misfolding and aggregation enabled by solid-state NMR spectroscopy.
Insights into protein misfolding and aggregation enabled by solid-state NMR spectroscopy.
Solid State Nucl Magn Reson. 2017 Oct 04;88:1-14
Authors: van der Wel PCA
Abstract
The aggregation of proteins and peptides into a variety of insoluble, and often non-native, aggregated states plays a central role in many devastating diseases. Analogous processes undermine the efficacy of polypeptide-based biological pharmaceuticals, but are also...
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10-17-2017 10:19 AM
Insights into protein misfolding and aggregation enabled by solid-state NMR spectroscopy
Insights into protein misfolding and aggregation enabled by solid-state NMR spectroscopy
Publication date: Available online 4 October 2017
Source:Solid State Nuclear Magnetic Resonance</br>
Author(s): Patrick C.A. van der Wel</br>
The aggregation of proteins and peptides into a variety of insoluble, and often non-native, aggregated states plays a central role in many devastating diseases. Analogous processes undermine the efficacy of polypeptide-based biological pharmaceuticals, but are also being leveraged in the design of biologically inspired self-assembling...
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10-05-2017 07:28 PM
[NMR paper] Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer
Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer
Once removed from their natural environment, membrane proteins depend on membrane-mimetic systems to retain their native structures and functions. To this end, lipid-bilayer nanodiscs that are bounded by scaffold proteins or amphiphilic polymers such as styrene/maleic acid (SMA) copolymers have been introduced as alternatives to detergent micelles and liposomes for in vitro membrane-protein research. Herein, we show that an alternating diisobutylene/maleic acid...
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01-12-2017 05:31 PM
[NMR paper] Optimizing nanodiscs and bicelles for solution NMR studies of two ?-barrel membrane proteins.
Optimizing nanodiscs and bicelles for solution NMR studies of two ?-barrel membrane proteins.
Related Articles Optimizing nanodiscs and bicelles for solution NMR studies of two ?-barrel membrane proteins.
J Biomol NMR. 2015 Apr;61(3-4):261-74
Authors: Kucharska I, Edrington TC, Liang B, Tamm LK
Abstract
Solution NMR spectroscopy has become a robust method to determine structures and explore the dynamics of integral membrane proteins. The vast majority of previous studies on membrane proteins by solution NMR have been conducted in...
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04-15-2015 04:40 PM
Optimizing nanodiscs and bicelles for solution NMR studies of two β-barrel membrane proteins
Optimizing nanodiscs and bicelles for solution NMR studies of two β-barrel membrane proteins
Abstract
Solution NMR spectroscopy has become a robust method to determine structures and explore the dynamics of integral membrane proteins. The vast majority of previous studies on membrane proteins by solution NMR have been conducted in lipid micelles. Contrary to the lipids that form a lipid bilayer in biological membranes, micellar lipids typically contain only a single hydrocarbon chain or two chains that are too short to form a bilayer. Therefore,...
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02-10-2015 10:56 AM
[Question from NMRWiki Q&A forum] How to catch Deviate Fish wow4.2
How to catch Deviate Fish wow4.2
4.2 map is changed, my fishing to 150, but in the Barrens that what fishing oasis in nearly an hour, a variation of the fish did not catch wow gold ah, who knows where to catch ah?
Check if somebody has answered this question on NMRWiki QA forum
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11-02-2011 02:29 PM
Nanodiscs versus Macrodiscs for NMR of Membrane Proteins
Nanodiscs versus Macrodiscs for NMR of Membrane Proteins
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi201289c/aop/images/medium/bi-2011-01289c_0002.gif
Biochemistry
DOI: 10.1021/bi201289c
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/4XXVT7tcC8w
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09-30-2011 08:01 PM
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Nanodiscs vs. Macrodiscs for NMR of Membrane Proteins.
Biochemistry. 2011 Sep 21;
Authors: Park SH, Berkamp S, Cook GA, Chan MK, Viadiu H, Opella SJ
Abstract
It is challenging to find membrane mimics that stabilize the native structure, dynamics, and functions of membrane proteins. In a recent advance, nanodiscs have been shown to provide a bilayer environment compatible with solution NMR. Increasing the lipid to "belt" peptide ratio expands their diameter, slows their reorientation...
Nanodiscs catch misfolding proteins - Xinhua
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