Mechanistic insights into allosteric regulation of the A2A adenosine G protein-coupled receptor by physiological cations - Nature.com

		BioNMR > NMR community > Online News
Mechanistic insights into allosteric regulation of the A2A adenosine G protein-coupled receptor by physiological cations - Nature.com

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-14-2024, 06:25 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Mechanistic insights into allosteric regulation of the A2A adenosine G protein-coupled receptor by physiological cations - Nature.com

Mechanistic insights into allosteric regulation of the A2A adenosine G protein-coupled receptor by physiological cations - Nature.com

Mechanistic insights into allosteric regulation of the A2A adenosine G protein-coupled receptor by physiological cations Nature.com Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor 19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor Abstract For the A2A adenosine receptor (A2AAR), a class A G-protein-coupled receptor (GPCR), reconstituted in n-dodecyl-Î²-D-maltoside (DDM)/â??â??â??â??â??cholesteryl hemisuccinate (CHS) mixed micelles, previous 19F-NMR studies revealed the presence of multiple simultaneously populated conformational states. Here, we study the influence of a different detergent, lauryl maltose neopentyl glycol (LMNG) in mixed micelles with CHS, and of lipid bilayer...	nmrlearner	Journal club	0	12-11-2023 11:33 PM
[NMR paper] 19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor 19F-NMR studies of the impact of different detergents and nanodiscs on the A2A adenosine receptor For the A(2A) adenosine receptor (A(2A)AR), a class A G-protein-coupled receptor (GPCR), reconstituted in n-dodecyl-?-D-maltoside (DDM)/?????cholesteryl hemisuccinate (CHS) mixed micelles, previous ^(19)F-NMR studies revealed the presence of multiple simultaneously populated conformational states. Here, we study the influence of a different detergent, lauryl maltose neopentyl glycol (LMNG) in mixed micelles with CHS, and of lipid bilayer nanodiscs on these conformational equilibria. The... ...	nmrlearner	Journal club	0	12-11-2023 11:33 PM
[NMR paper] Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy The photophysical properties of fluorescent proteins, including phototransformable variants used in advanced microscopy applications, are influenced by the environmental conditions in which they are expressed and used. Rational design of improved fluorescent protein markers requires a better understanding of these environmental effects. We demonstrate here that solution NMR spectroscopy can detect subtle changes in the chemical structure, conformation, and...	nmrlearner	Journal club	0	05-10-2021 04:54 PM
Cushing's syndrome driver mutation disrupts protein kinase A allosteric network, altering both regulation and substrate specificity - Science Advances Cushing's syndrome driver mutation disrupts protein kinase A allosteric network, altering both regulation and substrate specificity - Science Advances Cushing's syndrome driver mutation disrupts protein kinase A allosteric network, altering both regulation and substrate specificity Science Advances Read here	nmrlearner	Online News	0	02-29-2020 09:52 PM
[ASAP] Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor Matthew T. Eddy, Zhan-Guo Gao, Philip Mannes, Nilkanth Patel, Kenneth A. Jacobson, Vsevolod Katritch, Raymond C. Stevens, Kurt Wüthrich https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b03805/20180620/images/medium/ja-2018-03805n_0006.gif Journal of the American Chemical Society DOI: 10.1021/jacs.8b03805 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	06-21-2018 09:01 AM
[NMR paper] Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor. Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor. Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the A2A Adenosine Receptor. J Am Chem Soc. 2018 Jun 06;: Authors: Eddy MT, Gao ZG, Mannes P, Patel N, Jacobson KA, Katritch V, Stevens RC, Wüthrich K Abstract Tryptophan indole 15N-1H signals are well separated in nuclear magnetic resonance (NMR) spectra of proteins. Assignment of the indole 15N-1H signals...	nmrlearner	Journal club	0	06-07-2018 01:52 PM
Receptor Activity Modifying Proteins Have LimitedEffects on the Class B G Protein-Coupled Receptor Calcitonin Receptor-LikeReceptor Stalk Receptor Activity Modifying Proteins Have LimitedEffects on the Class B G Protein-Coupled Receptor Calcitonin Receptor-LikeReceptor Stalk http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b01180/20180207/images/medium/bi-2017-01180k_0008.gif Biochemistry DOI: 10.1021/acs.biochem.7b01180 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/8Z5smrN57aI More...	nmrlearner	Journal club	0	02-08-2018 04:02 AM
[NMR paper] Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk: insigh Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk: insights from NMR mapping studies and site-directed mutagenesis. Related Articles Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk: insights from NMR mapping studies and site-directed mutagenesis. J Mol Biol. 2001 Nov 16;314(1):129-38 Authors: Shekhtman A, Ghose R, Wang D, Cole PA, Cowburn D Csk (C-terminal Src kinase), a protein tyrosine kinase, consisting of the Src homology 2 and 3 (SH2 and SH3) domains and a catalytic domain,...	nmrlearner	Journal club	0	11-19-2010 08:44 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off