[NMR paper] Easy-to-Attach/Detach Solubilizing Tag-Aided Chemical Synthesis of an Aggregative Capsid Protein
Easy-to-Attach/Detach Solubilizing Tag-Aided Chemical Synthesis of an Aggregative Capsid Protein
A solubilizing "Trt-K10" tag was developed for the effective chemical preparation of peptides/proteins with low solubility. The Trt-K10 tag comprises a hydrophilic oligo-Lys sequence and a trityl anchor, and can be selectively introduced to a side chain thiol of Cys of deprotected peptides/proteins with a trityl alcohol-type introducing reagent "Trt(OH)-K10" under acidic conditions. Significantly, the ligation product in the reaction mixture of a thiol-additive-free native chemical ligation...
nmrlearner
Journal club
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01-09-2018 09:41 AM
[NMR images] making NMR invaluable as a
http://www.chem.duke.edu/~wwarren/fig83.jpg
http://www.chem.duke.edu/~wwarren/nmr.php
20/02/2012 3:31:02 PM GMT
making NMR invaluable as a
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nmrlearner
NMR pictures
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02-20-2012 03:25 PM
[KPWU blog] Making Ramachandran plot ?II
Making Ramachandran plot ?II
Long time ago, I made a blog article at here to make a record where I can go back to dig information of Ramachandran plot. But the ranges used in the Ramachandran plot are a bit out of date. The Richardson group has a publication in 2003*(Structure validation by Calpha geometry: phi,psi and Cbeta deviation http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=349&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
nmrlearner
News from NMR blogs
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04-20-2011 02:51 AM
[KPWU blog] [NMRPipe]?making a 3D cube
?making a 3D cube
On Feb 18th, an user asked whether there are ways to make a 3D cube of NMR data at the NMRPipe discussion group at Yahoo. Frank Delaglio, the author of NMRPipe, provided a very very nice script to generate the cube. The script is pasted in this post and user can copy it, save it http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=329&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
nmrlearner
News from NMR blogs
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04-15-2011 01:40 AM
[KPWU blog] PyMOL?making movies
PyMOL?making movies
I found several good online tutorials of making movies using PyMOL and provide the links at here. *I should make some examples by my own next time. PyMOL tips and tricks at Dept. Biochemistry, University of Cambridge How to make a movie PyMOL at Youtube Movie school #6 at PyMOL Wikihttp://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=319&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
nmrlearner
News from NMR blogs
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04-09-2011 12:17 AM
[NMR paper] Efficient analysis of protein 2D NMR spectra using the software package EASY.
Efficient analysis of protein 2D NMR spectra using the software package EASY.
Related Articles Efficient analysis of protein 2D NMR spectra using the software package EASY.
J Biomol NMR. 1991 Jul;1(2):111-30
Authors: Eccles C, Güntert P, Billeter M, Wüthrich K
The program EASY supports the spectral analysis of biomacromolecular two-dimensional (2D) nuclear magnetic resonance (NMR) data. It provides a user-friendly, window-based environment in which to view spectra for interactive interpretation. In addition, it includes a number of automated...
Making natural protein easy - New Food
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