3. (a) Give the basic principle involved in analytical centrifugation. (b) What are the important applications of this technique in biochemistry? 7. (a) Give the principle underlying in NMR spectrometry? (b) Describe the component of a NMR spectrometer with a neat labeled diagram.
Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Proteins. 2011 Mar;79(3):685-702
Authors: Webb H, Tynan-Connolly BM, Lee GM, Farrell D, O'Meara F, Søndergaard CR, Teilum K, Hewage C, McIntosh LP, Nielsen JE
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02-03-2011 01:44 PM
Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Remeasuring HEWL pK(a) values by NMR spectroscopy: Methods, analysis, accuracy, and implications for theoretical pK(a) calculations.
Proteins. 2010 Sep 17;
Authors: Webb H, Tynan-Connolly BM, Lee GM, Farrell D, O'Meara F, Søndergaard CR, Teilum K, Hewage C, McIntosh LP, Nielsen JE
Site-specific pK(a) values measured by NMR spectroscopy provide essential information on protein electrostatics, the pH-dependence of...
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12-25-2010 02:40 PM
[NMR paper] Inverse methods in two-dimensional NMR spectral analysis.
Inverse methods in two-dimensional NMR spectral analysis.
Related Articles Inverse methods in two-dimensional NMR spectral analysis.
J Magn Reson. 2003 May;162(1):141-57
Authors: van Beek JD, Meier BH, Schäfer H
Solid-state NMR is a valuable technique for the study of disordered materials. Analysis of such spectra usually involves solution of so-called ill-posed inverse problems. Here we present a strategy for the analysis of two-parameter two-dimensional NMR problems and test it on 2D DECODER and DOQSY experiments. Using Monte Carlo tests,...
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11-24-2010 09:01 PM
[NMR paper] Automated analysis of protein NMR assignments using methods from artificial intellige
Automated analysis of protein NMR assignments using methods from artificial intelligence.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Automated analysis of protein NMR assignments using methods from artificial intelligence.
J Mol Biol. 1997 Jun 20;269(4):592-610
Authors: Zimmerman DE, Kulikowski CA, Huang Y, Feng W, Tashiro M, Shimotakahara S, Chien C, Powers R, Montelione GT
An expert system for determining resonance assignments from NMR spectra of proteins is...
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08-22-2010 03:31 PM
[NMR paper] Automated analysis of protein NMR assignments using methods from artificial intellige
Automated analysis of protein NMR assignments using methods from artificial intelligence.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Automated analysis of protein NMR assignments using methods from artificial intelligence.
J Mol Biol. 1997 Jun 20;269(4):592-610
Authors: Zimmerman DE, Kulikowski CA, Huang Y, Feng W, Tashiro M, Shimotakahara S, Chien C, Powers R, Montelione GT
An expert system for determining resonance assignments from NMR spectra of proteins is...
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08-22-2010 03:03 PM
[NMR paper] Structural analysis of non-native states of proteins by NMR methods.
Structural analysis of non-native states of proteins by NMR methods.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural analysis of non-native states of proteins by NMR methods.
Curr Opin Struct Biol. 1996 Feb;6(1):24-30
Authors: Shortle DR
Established NMR methods are increasingly being applied to the non-native states of proteins. For small denatured proteins, full assignment of proton, 15N and 13C resonances is often straightforward. Sensitive methods exist...
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08-22-2010 02:27 PM
Structure-oriented methods for protein NMR data analysis
Structure-oriented methods for protein NMR data analysis
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 3 March 2010</br>
Guillermo A., Bermejo , Miguel, Llinás</br>
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08-16-2010 03:50 AM
EMBO NMR Practical Course 2008
2008 EMBO World Practical Course: Structure determination of biological macromolecules by solution NMR
Mon September 8 - Mon September 15 2008
The Beijing NMR Center, Peking University, Beijing, China
The intention of the course is to provide practical training in important aspects of structure determination by NMR. The emphasis will be on setting up experiments on the spectrometer (optimizing parameters, pulse programs), on processing and analyzing the NMR data and on structure calculations in a hands-on fashion. It is impossible to also teach all the necessary theoretical...