Inside a high-stakes experiment in protein crystallization - Phys.Org

		BioNMR > NMR community > Online News
Inside a high-stakes experiment in protein crystallization - Phys.Org

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-15-2015, 01:30 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Inside a high-stakes experiment in protein crystallization - Phys.Org

Phys.Org

Inside a high-stakes experiment in protein crystallization
Phys.Org
(The other is nuclear magnetic resonance spectroscopy.) Crystallography starts with crystalsâ??comprising hundreds of thousands of protein molecules, packed together like a three-dimensional wall of bricks. By bombarding a crystal with X-rays, and using ...

Inside a high-stakes experiment in protein crystallization - Phys.Org
More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Space Race: Inside a high-stakes experiment in protein crystallization - UAB News http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcTdLSDTqT9KHcynbDyRGfWeKnEzt1ralm67yDa3WwS2dRd9txZmssQcRxq5L2_rgLOrksdK31E UAB News <img alt="" height="1" width="1"> Space Race: Inside a high-stakes experiment in protein crystallization UAB News (The other is nuclear magnetic resonance spectroscopy.) Crystallography starts with crystals â?? comprising hundreds of thousands of protein molecules, packed together like a three-dimensional wall of bricks. By bombarding a crystal with X-rays, and ... Space Race: Inside a high-stakes experiment in protein crystallization - UAB...	nmrlearner	Online News	0	04-13-2015 09:11 PM
Researcher among best in protein modeling contests - Phys.Org Researcher among best in protein modeling contests - Phys.Org http://www.bionmr.com//t2.gstatic.com/images?q=tbn:ANd9GcQSlx694T61BoCaeKqyRR6QpXhWdpwgzkYKwLN94OV36EK6UX_U8E1jX8t2X9yG1IMz8IjVKafP Phys.Org <img alt="" height="1" width="1"> Researcher among best in protein modeling contests Phys.Org Kihara's work, called protein structure prediction, could shave years off of drug development and other work that depends on understanding protein structure and function. It also plays into the field of artificial protein design, where the future may ... and more »	nmrlearner	Online News	0	03-30-2015 06:04 PM
Understanding protein cross talk inside cells could lead to new breast cancer ... - Daily News - Galveston County Understanding protein cross talk inside cells could lead to new breast cancer ... - Daily News - Galveston County <img alt="" height="1" width="1" /> Understanding protein cross talk inside cells could lead to new breast cancer ... Daily News - Galveston County We are making progress in treatment and better diagnostics with advanced mammogram screening and the more refined NMR breast imaging. Together these are beginning to make a difference. And there are new promising techniques for specifically ... and more » Read here	nmrlearner	Online News	0	10-02-2014 06:36 AM
Protein courtship revealed through chemist's lens - Phys.Org Protein courtship revealed through chemist's lens - Phys.Org http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcQOKCXJUFn8JH3Fz7hNm0PQ2S3jQHg3sKDkrUjJ5Zd1vybPn96ocPBvEQrkW9nsxo85DzxQn7Ed Phys.Org <img alt="" height="1" width="1" /> Protein courtship revealed through chemist's lens Phys.Org But changes in the protein NMR spectra are small and weak, so finding out whether the trick worked wasn't straightforward. "At first, we didn't know what we were looking at. It appeared to us as uncertainty in the data, and it was extremely time ... and more »	nmrlearner	Online News	0	09-15-2014 07:13 PM
Measuring modified protein structures - Phys.Org <img alt="" height="1" width="1" /> Measuring modified protein structures Phys.Org Although there is a series of techniques to study structurally modified proteins, such as x-ray crystallography, nuclear magnetic resonance spectroscopy and other spectroscopic techniques, they cannot be used to analyse complex biological samples. and more » Measuring modified protein structures - Phys.Org More...	nmrlearner	Online News	0	09-15-2014 06:51 AM
New insight on HIV life cycle gained through creative use of high-energy X-rays - Phys.Org <img alt="" height="1" width="1" /> New insight on HIV life cycle gained through creative use of high-energy X-rays Phys.Org The structural basis of the recognition between RRE and Rev-protein remained elusive for many years because of the difficulties in the structural determination for the RRE RNA. Crystallography and nuclear magnetic resonance spectroscopy (NMR) are often ... New insight on HIV life cycle gained through creative use of high-energy X-rays - Phys.Org More...	nmrlearner	Online News	0	05-13-2014 03:11 PM
New player emerges in mapping protein structures - Phys.Org <img alt="" height="1" width="1" /> New player emerges in mapping protein structures Phys.Org Scientists also use nuclear magnetic resonance imaging to solve structures. And coming in at a distant third is a technique called single-particle cryoelectron microscopy (cryo-EM), in which a macromolecule is immobilized in a thin film of vitrified ... and more » New player emerges in mapping protein structures - Phys.Org More...	nmrlearner	Online News	0	09-20-2013 02:39 PM
[NMR paper] Multidimensional NMR spectroscopy for protein characterization and assignment inside cells. Multidimensional NMR spectroscopy for protein characterization and assignment inside cells. Related Articles Multidimensional NMR spectroscopy for protein characterization and assignment inside cells. J Am Chem Soc. 2005 Aug 10;127(31):10848-9 Authors: Reardon PN, Spicer LD High-field, heteronuclear NMR spectroscopy of biological macromolecules in native cellular environments is limited by the low concentrations present and the long data acquisition times needed for the experiments. Successful 1D and 2D heteronuclear NMR data have been...	nmrlearner	Journal club	0	12-01-2010 06:56 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off