[NMR paper] Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
Solid-state NMR (ssNMR) spectroscopy facilitates the non-destructive characterization of structurally heterogeneous biomolecules in their native setting, e.g., comprising proteins, lipids and polysaccharides. Here we demonstrate the utility of high and ultra-high field 1H-detected fast MAS ssNMR spectroscopy, which exhibits increased sensitivity and spectral resolution to further elucidate the atomic-level composition and structural arrangement of the cell wall of...
nmrlearner
Journal club
0
10-02-2022 07:47 AM
[NMR paper] Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell...
nmrlearner
Journal club
0
03-30-2021 08:29 AM
[NMR paper] Erratum to: Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Erratum to: Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Related Articles Erratum to: Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
J Biomol NMR. 2017 Dec 11;:
Authors: Takeuchi K, Arthanari H, Shimada I, Wagner G
Abstract
The authors regret a mistake appeared in the supplement of this paper.
[NMR paper] Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Related Articles Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
J Biomol NMR. 2015 Oct 23;
Authors: Takeuchi K, Arthanari H, Shimada I, Wagner G
Abstract
Detection of (15)N in multidimensional NMR experiments of proteins has sparsely been utilized because of the low gyromagnetic ratio (?) of nitrogen and the presumed low sensitivity of such experiments. Here we show that selecting the TROSY...
nmrlearner
Journal club
0
10-27-2015 12:33 PM
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR
Abstract
Detection of 15N in multidimensional NMR experiments of proteins has sparsely been utilized because of the low gyromagnetic ratio (γ) of nitrogen and the presumed low sensitivity of such experiments. Here we show that selecting the TROSY components of proton-attached 15N nuclei (TROSY 15NH) yields high quality spectra in high field magnets (>600Â*MHz) by taking advantage of the slow 15N transverse relaxation and compensating for the inherently low...
High resolution protein in-cell NMR in zebrafish embryos - ScienceDirect.com
Linear Mode
