Fold it: Solid-state NMR unravels proteins spectroscopyNOW.com
Researchers have developed a novel solid-state NMR spectroscopic method that uses paramagnetic tags to help them visualize the shape of protein molecules. The technique could be used to help scientists understand the properties of various biological ...
Solid-state NMR of proteins sedimented by ultracentrifugation [Chemistry]
Solid-state NMR of proteins sedimented by ultracentrifugation
Bertini, I., Luchinat, C., Parigi, G., Ravera, E., Reif, B., Turano, P....
Date: 2011-06-28
Relatively large proteins in solution, spun in NMR rotors for solid samples at typical ultracentrifugation speeds, sediment at the rotor wall. The sedimented proteins provide high-quality solid-state-like NMR spectra suitable for structural investigation. The proteins fully revert to the native solution state when spinning is stopped, allowing one to study them in both conditions. Transiently sedimented proteins can be considered a...
nmrlearner
Journal club
0
06-29-2011 04:45 AM
Solid-state NMR of proteins sedimented by ultracentrifugation.
Solid-state NMR of proteins sedimented by ultracentrifugation.
Solid-state NMR of proteins sedimented by ultracentrifugation.
Proc Natl Acad Sci U S A. 2011 Jun 13;
Authors: Bertini I, Luchinat C, Parigi G, Ravera E, Reif B, Turano P
Relatively large proteins in solution, spun in NMR rotors for solid samples at typical ultracentrifugation speeds, sediment at the rotor wall. The sedimented proteins provide high-quality solid-state-like NMR spectra suitable for structural investigation. The proteins fully revert to the native solution state when...
nmrlearner
Journal club
0
06-15-2011 01:15 PM
[NMR paper] Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes.
Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes.
Related Articles Solid-state 17O NMR as a probe for structural studies of proteins in biomembranes.
J Am Chem Soc. 2004 Dec 1;126(47):15320-1
Authors: Lemaître V, de Planque MR, Howes AP, Smith ME, Dupree R, Watts A
We report the first example of 17O NMR spectra from a selectively labeled transmembrane peptide, 17O--WALP23, as a lyophilized powder and incorporated in hydrated phospholipid vesicles. It is shown that at high magnetic field it is feasible to apply...
nmrlearner
Journal club
0
11-24-2010 10:03 PM
[NMR paper] Solid-state NMR studies of the structure and mechanisms of proteins.
Solid-state NMR studies of the structure and mechanisms of proteins.
Related Articles Solid-state NMR studies of the structure and mechanisms of proteins.
Curr Opin Struct Biol. 2002 Oct;12(5):661-9
Authors: Thompson LK
Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the development...
nmrlearner
Journal club
0
11-24-2010 08:58 PM
[NMR paper] Solid-state NMR spectroscopy applied to membrane proteins.
Solid-state NMR spectroscopy applied to membrane proteins.
Related Articles Solid-state NMR spectroscopy applied to membrane proteins.
Curr Opin Struct Biol. 2000 Oct;10(5):593-600
Authors: de Groot HJ
One major remaining problem in structural biology is to elucidate the structure and mechanism of function of membrane proteins. On the basis of preliminary information from genome projects, it is now estimated that up to 50,000 different membrane proteins may exist in the human being and that virtually every life process proceeds, sooner or...
nmrlearner
Journal club
0
11-19-2010 08:29 PM
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
J Biomol NMR. 2010 Oct 30;
Authors: Trent Franks W, Atreya HS, Szyperski T, Rienstra CM
Recording of four-dimensional (4D) spectra for proteins in the solid state has opened new avenues to obtain virtually complete resonance assignments and three-dimensional (3D) structures of proteins. As in solution state NMR, the sampling of three indirect dimensions leads per se to long minimal measurement time. Furthermore,...
nmrlearner
Journal club
0
11-06-2010 11:02 AM
[NMR paper] Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study.
Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study.
Related Articles Dynamic structure of proteins in solid state. 1H and 13C NMR relaxation study.
J Biomol Struct Dyn. 1996 Oct;14(2):211-24
Authors: Krushelnitsky AG, Fedotov VD, Spevacek J, Straka J
Temperature dependencies of 1H non-selective NMR T1 and T2 relaxation times measured at two resonance frequencies and natural abundance 13C NMR relaxation times T1 and T1r measured at room temperature have been studied in a set of dry and wet solid proteins - Bacterial...
nmrlearner
Journal club
0
08-22-2010 02:20 PM
Solid State NMR of membrane peptides and proteins
Solid State NMR of membrane peptides and proteins
Lecture notes on "Solid State NMR of membrane peptides and proteins" by Dr. SK Straus from Univ. of British Columbia
More...
Fold it: Solid-state NMR unravels proteins - spectroscopyNOW.com
Linear Mode
