[NMR paper] Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach.
Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach.
Related Articles Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach.
J Biomol NMR. 2016 Jan 19;
Authors: Wang S, Matsuda I, Long F, Ishii Y
Abstract
This study demonstrates a novel spectral editing technique for protein solid-state NMR (SSNMR) to simplify the spectrum drastically and to reduce...
nmrlearner
Journal club
0
01-20-2016 11:54 PM
Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach
Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach
Abstract
This study demonstrates a novel spectral editing technique for protein solid-state NMR (SSNMR) to simplify the spectrum drastically and to reduce the ambiguity for protein main-chain signal assignments in fast magic-angle-spinning (MAS) conditions at a wide frequency range of 40â??80Â*kHz. The approach termed HIGHLIGHT (Wang et al., in Chem Comm 51:15055â??15058, 2015) combines the reverse 13C,...
nmrlearner
Journal club
0
01-19-2016 07:37 PM
[NMR paper] Effortless assignment with 4D covariance sequential correlation maps
Effortless assignment with 4D covariance sequential correlation maps
Publication date: Available online 21 September 2015
Source:Journal of Magnetic Resonance</br>
Author(s): Bradley J. Harden, Subrata H. Mishra, Dominique P. Frueh</br>
Traditional Nuclear Magnetic Resonance (NMR) assignment procedures for proteins rely on preliminary peak-picking to identify and label NMR signals. However, such an approach has severe limitations when signals are erroneously labeled or completely neglected. The consequences are especially grave for proteins with substantial...
nmrlearner
Journal club
0
09-22-2015 05:38 AM
[NMR paper] Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives.
Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives.
Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives.
J Am Chem Soc. 2014 Sep 16;
Authors: Harden BJ, Nichols SR, Frueh DP
Abstract
Nuclear magnetic resonance (NMR) studies of larger proteins are hampered by difficulties in assigning NMR resonances. Human intervention is typically required to identify NMR signals in 3D spectra, and subsequent procedures...
nmrlearner
Journal club
0
09-17-2014 11:54 AM
FacilitatedAssignment of Large Protein NMR Signalswith Covariance Sequential Spectra Using Spectral Derivatives
FacilitatedAssignment of Large Protein NMR Signalswith Covariance Sequential Spectra Using Spectral Derivatives
Bradley J. Harden, Scott R. Nichols and Dominique P. Frueh
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja5058407/20140916/images/medium/ja-2014-058407_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja5058407
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/nO-MaipYTjs
nmrlearner
Journal club
0
09-16-2014 08:38 PM
Spectral editing of two-dimensional magic-angle-spinning solid-state NMR spectra for protein resonance assignment and structure determination
Spectral editing of two-dimensional magic-angle-spinning solid-state NMR spectra for protein resonance assignment and structure determination
Abstract Several techniques for spectral editing of 2D 13Câ??13C correlation NMR of proteins are introduced. They greatly reduce the spectral overlap for five common amino acid types, thus simplifying spectral assignment and conformational analysis. The carboxyl (COO) signals of glutamate and aspartate are selected by suppressing the overlapping amide Nâ??CO peaks through 13Câ??15N dipolar dephasing. The sidechain methine (CH) signals of valine,...
nmrlearner
Journal club
0
10-13-2012 04:42 AM
[NMR paper] Sequential assignment of 2D-NMR spectra of proteins using genetic algorithms.
Sequential assignment of 2D-NMR spectra of proteins using genetic algorithms.
Related Articles Sequential assignment of 2D-NMR spectra of proteins using genetic algorithms.
J Chem Inf Comput Sci. 1993 Mar-Apr;33(2):245-51
Authors: Wehrens R, Lucasius C, Buydens L, Kateman G
The application of genetic algorithms to the problem of the sequential assignment of two-dimensional protein NMR spectra is discussed. The problem is heavily underconstrained since in most cases more patterns are available than amino acid positions, and uncertainties may...
nmrlearner
Journal club
0
08-21-2010 11:53 PM
[NMR paper] A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: h
A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.
Related Articles A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.
Biochemistry. 1990 May 15;29(19):4659-67
Authors: Ikura M, Kay LE, Bax A
A novel approach is described for obtaining sequential assignment of the backbone 1H, 13C, and 15N...
Facilitated Assignment of Large Protein NMR Signals with Covariance Sequential Spectra Using Spectral Derivatives - ACS Publications
Linear Mode
