Efficient production of a functional G protein-coupled receptor in E. coli for structural studies
Efficient production of a functional G protein-coupled receptor in E. coli for structural studies
Abstract
G protein-coupled receptors (GPCRs) are transmembrane signal transducers which regulate many key physiological process. Since their discovery, their analysis has been limited by difficulties in obtaining sufficient amounts of the receptors in high-quality, functional form from heterologous expression hosts. Albeit highly attractive because of its simplicity and the ease of isotope labeling for NMR studies, heterologous expression of functional...
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[NMR paper] Expression, Functional Characterization, and Solid-State NMR Investigation of the G Protein-Coupled GHS Receptor in Bilayer Membranes.
Expression, Functional Characterization, and Solid-State NMR Investigation of the G Protein-Coupled GHS Receptor in Bilayer Membranes.
Expression, Functional Characterization, and Solid-State NMR Investigation of the G Protein-Coupled GHS Receptor in Bilayer Membranes.
Sci Rep. 2017 Apr 07;7:46128
Authors: Schrottke S, Kaiser A, Vortmeier G, Els-Heindl S, Worm D, Bosse M, Schmidt P, Scheidt HA, Beck-Sickinger AG, Huster D
Abstract
The expression, functional reconstitution and first NMR characterization of the human growth...
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04-08-2017 10:57 AM
[NMR paper] NMR Investigation of Structures of G-Protein Coupled Receptor Folding Intermediates.
NMR Investigation of Structures of G-Protein Coupled Receptor Folding Intermediates.
Related Articles NMR Investigation of Structures of G-Protein Coupled Receptor Folding Intermediates.
J Biol Chem. 2016 Nov 18;:
Authors: Poms M, Ansorge P, Martinez-Gil L, Jurt S, Gottstein D, Fracchiolla KE, Cohen LS, Guentert P, Mingarro I, Naider F, Zerbe O
Abstract
Folding of G-protein coupled receptors (GPCRs) according to the two-stage model (Popot et al., Biochemistry 29(1990), 4031) is postulated to proceed in 2 steps: Partitioning of...
[NMR paper] The dynamics of the G protein-coupled neuropeptide Y2 receptor in monounsaturated membranes investigated by solid-state NMR spectroscopy.
The dynamics of the G protein-coupled neuropeptide Y2 receptor in monounsaturated membranes investigated by solid-state NMR spectroscopy.
The dynamics of the G protein-coupled neuropeptide Y2 receptor in monounsaturated membranes investigated by solid-state NMR spectroscopy.
J Biomol NMR. 2015 Jan 6;
Authors: Thomas L, Kahr J, Schmidt P, Krug U, Scheidt HA, Huster D
Abstract
In contrast to the static snapshots provided by protein crystallography, G protein-coupled receptors constitute a group of proteins with highly dynamic...
[NMR paper] G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy.
G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy.
G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy.
Biochem J. 2013 Mar 15;450(3):443-57
Authors: Ding X, Zhao X, Watts A
Abstract
GPCRs (G-protein-coupled receptors) are versatile signalling molecules at the cell surface and make up the largest and most diverse family of membrane receptors in the human genome. They convert a large variety of extracellular stimuli into...
Expression, Functional Characterization, and Solid-State NMR Investigation of the G Protein-Coupled GHS Receptor in ... - Nature.com
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