Enhanced-sensitivity NMR could reveal new clues to how proteins fold - Phys.Org

		BioNMR > NMR community > Online News
Enhanced-sensitivity NMR could reveal new clues to how proteins fold - Phys.Org

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-09-2015, 04:49 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,778

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Enhanced-sensitivity NMR could reveal new clues to how proteins fold - Phys.Org

Enhanced-sensitivity NMR could reveal new clues to how proteins fold - Phys.Org

Phys.Org

Enhanced-sensitivity NMR could reveal new clues to how proteins fold
Phys.Org
Until now, it has been difficult to fully characterize the different structures that proteins can take on in their natural environments. However, using a new technique known as sensitivity-enhanced nuclear magnetic resonance (NMR), MIT researchers have ...

and more »
Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus Publication date: Available online 8 October 2015 Source:Cell</br> Author(s): KendraK. Frederick, VladimirK. Michaelis, Björn Corzilius, Ta-Chung Ong, AngelaC. Jacavone, RobertG. Griffin, Susan Lindquist</br> Biological processes occur in complex environments containing a myriad of potential interactors. Unfortunately, limitations on the sensitivity of biophysical techniques normally restrict structural investigations to purified systems, at concentrations that are...	nmrlearner	Journal club	0	10-09-2015 03:05 AM
One hundred fold overall sensitivity enhancements for Silicon-29 NMR spectroscopy of surfaces by dynamic nuclear polarization with CPMG acquisition From The DNP-NMR Blog: One hundred fold overall sensitivity enhancements for Silicon-29 NMR spectroscopy of surfaces by dynamic nuclear polarization with CPMG acquisition Rossini, A.J., et al., One hundred fold overall sensitivity enhancements for Silicon-29 NMR spectroscopy of surfaces by dynamic nuclear polarization with CPMG acquisition. Chemical Science, 2012. 3(1): p. 108-115. http://dx.doi.org/10.1039/C1SC00550B	nmrlearner	News from NMR blogs	0	05-15-2013 03:12 PM
A trick to fold proteins more quickly - PhysOrg.com - Phys.Org <img alt="" height="1" width="1" /> A trick to fold proteins more quickly - PhysOrg.com Phys.Org "We exploit the experimental data obtained observing the proteins through nuclear magnetic resonance, and use them to create restraints to be applied to the model", explained Laio, who has coordinated the research published in Proceedings of the ... A trick to fold proteins more quickly - PhysOrg.com - Phys.Org More...	nmrlearner	Online News	0	05-08-2013 02:49 PM
Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold. Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold. Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold. J Struct Funct Genomics. 2010 Dec 14; Authors: Mani R, Vorobiev S, Swapna GV, Neely H, Janjua H, Ciccosanti C, Xiao R, Acton TB, Everett JK, Hunt J, Montelione GT The conserved Lipoprotein-17 domain of membrane-associated protein Q9PRA0_UREPA from Ureaplasma parvum was selected for structure determination by the Northeast Structural...	nmrlearner	Journal club	0	12-15-2010 12:03 PM
[NMR paper] NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyas NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyase involved in the bacterial mercury resistance system. Related Articles NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyase involved in the bacterial mercury resistance system. Biochemistry. 2004 Jul 6;43(26):8322-32 Authors: Di Lello P, Benison GC, Valafar H, Pitts KE, Summers AO, Legault P, Omichinski JG Mercury resistant bacteria have developed a system of two enzymes (MerA and MerB), which allows them to efficiently...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection. Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection. Related Articles Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection. J Magn Reson. 2001 May;150(1):43-8 Authors: Hong M, Yamaguchi S A method for enhancing the sensitivity of 15N spectra of nonspinning solids through 1H indirect detection is introduced. By sampling the 1H signals in the windows of a pulsed spin-lock sequence, high-sensitivity 1H spectra can be obtained in two-dimensional (2D) spectra whose indirect dimension yields the 15N chemical...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxatio Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins. Related Articles Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins. J Biomol NMR. 2000 May;17(1):55-61 Authors: Liu A, Hu W, Qamar S, Majumdar A In this paper, we demonstrate that the sensitivity of triple-resonance NMR experiments can be enhanced...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[NMR paper] Enhanced protein fold recognition using secondary structure information from NMR. Enhanced protein fold recognition using secondary structure information from NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Enhanced protein fold recognition using secondary structure information from NMR. Protein Sci. 1999 May;8(5):1127-33 Authors: Ayers DJ, Gooley PR, Widmer-Cooper A, Torda AE NMR offers...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off