Enhanced picture of protein-folding intermediates using organic solvents in H/D exchange and quench-flow experiments - pnas.org

		BioNMR > NMR community > Online News
Enhanced picture of protein-folding intermediates using organic solvents in H/D exchange and quench-flow experiments - pnas.org

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-30-2024, 01:20 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,731

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Enhanced picture of protein-folding intermediates using organic solvents in H/D exchange and quench-flow experiments - pnas.org

Enhanced picture of protein-folding intermediates using organic solvents in H/D exchange and quench-flow experiments - pnas.org

Enhanced picture of protein-folding intermediates using organic solvents in H/D exchange and quench-flow experiments pnas.org Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and - pnas.org Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and - pnas.org Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and pnas.org Read here	nmrlearner	Online News	0	07-31-2024 03:41 AM
Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and - pnas.org Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and - pnas.org Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and pnas.org Read here	nmrlearner	Online News	0	07-24-2024 08:25 AM
Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and 19F ... - pnas.org Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and 19F ... - pnas.org Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and 19F ... pnas.org Read here	nmrlearner	Online News	0	02-23-2024 11:21 PM
[NMR thesis] I. Studies on Cycloproply-Stabilized Vinyl Cation Intermediates. II. Molecular Orbital Calculations on Cationic Intermediates and Displacement Reactions. III. On the Complexation of NMR Lanthanide Shift Reagents with Organic Substrates I. Studies on Cycloproply-Stabilized Vinyl Cation Intermediates. II. Molecular Orbital Calculations on Cationic Intermediates and Displacement Reactions. III. On the Complexation of NMR Lanthanide Shift Reagents with Organic Substrates Kelsey, Donald Ross (1973) I. Studies on Cycloproply-Stabilized Vinyl Cation Intermediates. II. Molecular Orbital Calculations on Cationic Intermediates and Displacement Reactions. III. On the Complexation of NMR Lanthanide Shift Reagents with Organic Substrates. Dissertation (Ph.D.), California Institute of Technology. ...	nmrlearner	NMR theses	0	11-25-2018 06:02 AM
[NMR paper] A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis Available online 5 March 2013 Publication year: 2013 Source:Journal of Magnetic Resonance</br> </br> Rapid freeze quench electron paramagnetic resonance (RFQ)-EPR is a method for trapping short lived intermediates in chemical reactions and subjecting them to EPR spectroscopy investigation for their characterization. Two (or more) reacting components are mixed at room temperature and after some delay the mixture is sprayed into a cold trap and transferred into...	nmrlearner	Journal club	0	03-06-2013 04:33 PM
NMR as a tool to identify and characterize protein folding intermediates NMR as a tool to identify and characterize protein folding intermediates Available online 12 September 2012 Publication year: 2012 Source:Archives of Biochemistry and Biophysics</br> </br> NMR spectroscopy is one of the few biophysical methods that can provide atomic-level insight into the conformation of partially folded states and/or intermediates present along the protein folding pathway. Such studies are important not only within the context of the protein folding problem, but also to push forward the technique, due to the challenging nature of the systems studied....	nmrlearner	Journal club	0	02-03-2013 10:13 AM
[NMR paper] Rapid amide proton exchange rates in peptides and proteins measured by solvent quench Rapid amide proton exchange rates in peptides and proteins measured by solvent quenching and two-dimensional NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Rapid amide proton exchange rates in peptides and proteins measured by solvent quenching and two-dimensional NMR. Protein Sci. 1995 Apr;4(4):804-14 Authors: Zhang YZ,...	nmrlearner	Journal club	0	08-22-2010 03:41 AM
[NMR paper] NMR and protein folding: equilibrium and stopped-flow studies. NMR and protein folding: equilibrium and stopped-flow studies. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR and protein folding: equilibrium and stopped-flow studies. Protein Sci. 1993 Dec;2(12):2007-14 Authors: Frieden C, Hoeltzli SD, Ropson IJ NMR studies are now unraveling the structure of intermediates...	nmrlearner	Journal club	0	08-22-2010 03:01 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off