Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation | Proceedings of the National Academy of Sciences - pnas.org

		BioNMR > NMR community > Online News
Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation \| Proceedings of the National Academy of Sciences - pnas.org

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-04-2022, 10:35 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation | Proceedings of the National Academy of Sciences - pnas.org

Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation | Proceedings of the National Academy of Sciences - pnas.org

Dissecting the stability determinants of a challenging de novo protein fold using massively parallel design and experimentation | Proceedings of the National Academy of Sciences pnas.org Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase \| Proceedings of the National Academy of Sciences - pnas.org Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase \| Proceedings of the National Academy of Sciences - pnas.org Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	09-15-2022 06:02 AM
Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase \| Proceedings of the National Academy of Sciences - pnas.org Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase \| Proceedings of the National Academy of Sciences - pnas.org Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	09-07-2022 04:33 AM
A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein \| Proceedings of the National Academy of Sciences - pnas.org A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein \| Proceedings of the National Academy of Sciences - pnas.org A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	08-26-2022 04:47 AM
De novo designed protein inhibitors of amyloid aggregation and seeding \| Proceedings of the National Academy of Sciences - pnas.org De novo designed protein inhibitors of amyloid aggregation and seeding \| Proceedings of the National Academy of Sciences - pnas.org De novo designed protein inhibitors of amyloid aggregation and seeding \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	08-24-2022 02:40 PM
Vibrational couplings between protein and cofactor in bacterial phytochrome Agp1 revealed by 2D-IR spectroscopy \| Proceedings of the National Academy of Sciences - pnas.org Vibrational couplings between protein and cofactor in bacterial phytochrome Agp1 revealed by 2D-IR spectroscopy \| Proceedings of the National Academy of Sciences - pnas.org Vibrational couplings between protein and cofactor in bacterial phytochrome Agp1 revealed by 2D-IR spectroscopy \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	08-04-2022 01:03 AM
A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein \| Proceedings of the National Academy of Sciences - pnas.org A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein \| Proceedings of the National Academy of Sciences - pnas.org A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	06-29-2022 05:34 AM
Design principles of PI(4,5)P2 clustering under protein-free conditions: Specific cation effects and calcium-potassium synergy \| Proceedings of the National Academy of Sciences - pnas.org Design principles of PI(4,5)P2 clustering under protein-free conditions: Specific cation effects and calcium-potassium synergy \| Proceedings of the National Academy of Sciences - pnas.org Design principles of PI(4,5)P2 clustering under protein-free conditions: Specific cation effects and calcium-potassium synergy \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	06-24-2022 10:32 AM
Multi-eGO: An in silico lens to look into protein aggregation kinetics at atomic resolution \| Proceedings of the National Academy of Sciences - pnas.org Multi-eGO: An in silico lens to look into protein aggregation kinetics at atomic resolution \| Proceedings of the National Academy of Sciences - pnas.org Multi-eGO: An in silico lens to look into protein aggregation kinetics at atomic resolution \| Proceedings of the National Academy of Sciences pnas.org Read here	nmrlearner	Online News	0	06-23-2022 06:52 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off