[NMR paper] Activity-Based Probes Developed by Applying Sequential Dehydroalanine Strategy on Expressed Proteins Reveal a Potential ?-globin Modulating Deubiquitinase
Activity-Based Probes Developed by Applying Sequential Dehydroalanine Strategy on Expressed Proteins Reveal a Potential ?-globin Modulating Deubiquitinase
We report a general and novel semisynthetic strategy for the preparation of ubiquitinated protein activity based probes applying sequential dehydroalanine formation on expressed proteins. We applied this approach to construct physiologically and therapeutically relevant ubiquitinated ?-globin probe, which was used for the enrichment and proteomic identification of ?-globin modulating deubiquitinases. We found USP15 as a potential...
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03-12-2018 02:28 PM
At last, cancer-fighting inhibitors hit deubiquitinase target selectively ... - The Biological SCENE
http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcSlwrRUX7xpKKGSxiyVp-aDOn7FaGqLt1se2PdgSOeSxwFGBqwI7vwb_JUFZ9UCSr0B2gZNF68
The Biological SCENE
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At last, cancer-fighting inhibitors hit deubiquitinase target selectively ...
The Biological SCENE
In the body, ubiquitinating enzymes regulate proteinsâ??marking them for disposal, for exampleâ??by adding ubiquitin peptides to them. And deubiquitinases ...
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At last, cancer-fighting inhibitors hit deubiquitinase target selectively ... - The Biological SCENE
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10-22-2017 04:54 PM
[NMR paper] Proximity-triggered covalent stabilization of low-affinity protein complexes in vitro and in vivo
Proximity-triggered covalent stabilization of low-affinity protein complexes in vitro and in vivo
The characterization of low-affinity protein complexes is challenging due to their dynamic nature. Here we present a method to stabilize transient protein complexes in vivo by generating a covalent and conformationally flexible bridge between the interaction partners. A highly active pyrrolysyl tRNA synthetase mutant directs the incorporation of unnatural amino acids bearing bromoalkyl moieties (BrCnK) into proteins. We demonstrate for the first time that low-affinity protein complexes...
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09-28-2017 07:57 PM
Conserved SecA Signal Peptide-Binding Site Revealedby Engineered Protein Chimeras and Fo?rster Resonance EnergyTransfer
Conserved SecA Signal Peptide-Binding Site Revealedby Engineered Protein Chimeras and Fo?rster Resonance EnergyTransfer
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b01115/20160219/images/medium/bi-2015-011152_0005.gif
Biochemistry
DOI: 10.1021/acs.biochem.5b01115
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/v7K1b408Q1s
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02-20-2016 11:00 AM
Human–Mouse Chimeras with Normal Expressionand Function Reveal That Major Domain Swapping Is Tolerated by P-Glycoprotein(ABCB1)
Human–Mouse Chimeras with Normal Expressionand Function Reveal That Major Domain Swapping Is Tolerated by P-Glycoprotein(ABCB1)
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b01064/20160210/images/medium/bi-2015-01064e_0010.gif
Biochemistry
DOI: 10.1021/acs.biochem.5b01064
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/zhYScDUkhL8
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02-11-2016 10:03 AM
[NMR paper] Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.
Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.
Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.
J Biomol NMR. 2013 Sep 7;
Authors: Tumulka F, Roos C, Löhr F, Bock C, Bernhard F, Dötsch V, Abele R
Abstract
The ATP binding cassette transporter TAPL translocates cytosolic peptides into the lumen of lysosomes driven by the hydrolysis of ATP. Functionally, this transporter can be...
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09-10-2013 08:44 PM
[NMR paper] Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dy
Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
Related Articles Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
Eur J Biochem. 2003 Dec;270(23):4606-14
Authors: Lamosa P, Turner DL, Ventura R, Maycock C, Santos H
Heteronuclear NMR relaxation measurements and hydrogen exchange data have been used to characterize protein dynamics in the presence or absence of...
Deubiquitinase-targeting chimeras for targeted protein stabilization - Nature.com
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