Determination of the geometric structure of the metal site in a blue copper protein by paramagnetic NMR - pnas.org

		BioNMR > NMR community > Online News
Determination of the geometric structure of the metal site in a blue copper protein by paramagnetic NMR - pnas.org

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-10-2024, 01:23 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Determination of the geometric structure of the metal site in a blue copper protein by paramagnetic NMR - pnas.org

Determination of the geometric structure of the metal site in a blue copper protein by paramagnetic NMR - pnas.org

Determination of the geometric structure of the metal site in a blue copper protein by paramagnetic NMR pnas.org Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Protein Structure Determination by Conformational Space Annealing using NMR Geometric Restraints Protein Structure Determination by Conformational Space Annealing using NMR Geometric Restraints Publication date: 16 February 2016 Source:Biophysical Journal, Volume 110, Issue 3, Supplement 1</br> Author(s): Jooyoung Lee, Keehyoung Joo, InSuk Joung, Jinhyuk Lee, Jinwoo Lee, Weontae Lee, Bernard Brooks, Sung Jong Lee</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	02-17-2016 07:50 PM
[NMR paper] Protein structure determination by conformational space annealing using NMR geometric restraints. Protein structure determination by conformational space annealing using NMR geometric restraints. Related Articles Protein structure determination by conformational space annealing using NMR geometric restraints. Proteins. 2015 Oct 10; Authors: Joo K, Joung I, Lee J, Lee J, Lee W, Brooks B, Lee SJ, Lee J Abstract We have carried out numerical experiments to investigate the applicability of the global optimization method of conformational space annealing (CSA) to the enhanced NMR protein structure determination over existing PDB...	nmrlearner	Journal club	0	10-12-2015 01:04 AM
[NMR paper] Metal-ligand interactions in perturbed blue copper sites: a paramagnetic (1)H NMR stu Metal-ligand interactions in perturbed blue copper sites: a paramagnetic (1)H NMR study of Co(II)-pseudoazurin. Related Articles Metal-ligand interactions in perturbed blue copper sites: a paramagnetic (1)H NMR study of Co(II)-pseudoazurin. J Biol Inorg Chem. 2003 Jan;8(1-2):75-82 Authors: Fernández CO, Niizeki T, Kohzuma T, Vila AJ Pseudoazurin is an electron transfer copper protein, a member of the cupredoxin family. The protein is frequently found in denitrifying bacteria, where it is the electron donor of nitrite reductase. The copper at...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] Metal-ligand interplay in blue copper proteins studied by 1H NMR spectroscopy: Cu(II) Metal-ligand interplay in blue copper proteins studied by 1H NMR spectroscopy: Cu(II)-pseudoazurin and Cu(II)-rusticyanin. Related Articles Metal-ligand interplay in blue copper proteins studied by 1H NMR spectroscopy: Cu(II)-pseudoazurin and Cu(II)-rusticyanin. J Am Chem Soc. 2002 Nov 20;124(46):13698-708 Authors: Donaire A, Jiménez B, Fernández CO, Pierattelli R, Niizeki T, Moratal JM, Hall JF, Kohzuma T, Hasnain SS, Vila AJ The blue copper proteins (BCPs), pseudoazurin from Achromobacter cycloclastes and rusticyanin from Thiobacillus...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] Electronic characterization of the oxidized state of the blue copper protein rusticya Electronic characterization of the oxidized state of the blue copper protein rusticyanin by 1H NMR: is the axial methionine the dominant influence for the high redox potential? Related Articles Electronic characterization of the oxidized state of the blue copper protein rusticyanin by 1H NMR: is the axial methionine the dominant influence for the high redox potential? Biochemistry. 2001 Jan 23;40(3):837-46 Authors: Donaire A, Jiménez B, Moratal J, Hall JF, Hasnain SS The oxidized state of rusticyanin, the blue copper protein with the highest...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Selective observation of the Cu(I)-amicyanin metal site by paramagnetic NMR on partia Selective observation of the Cu(I)-amicyanin metal site by paramagnetic NMR on partially oxidised samples. Selective observation of the Cu(I)-amicyanin metal site by paramagnetic NMR on partially oxidised samples. J Biomol NMR. 1997 Apr;9(3):299-305 Authors: Salgado J, Kalverda AP, Canters GW The relaxation enhancement caused by paramagnetic copper(II) is used to observe selectivelythe metal site of copper(I)-amicyanin by one- and two-dimensional NMR spectroscopy. Theparamagnetic effect is communicated to the diamagnetic protein through the...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Selective observation of the Cu(I)-amicyanin metal site by paramagnetic NMR on partia Selective observation of the Cu(I)-amicyanin metal site by paramagnetic NMR on partially oxidised samples. Selective observation of the Cu(I)-amicyanin metal site by paramagnetic NMR on partially oxidised samples. J Biomol NMR. 1997 Apr;9(3):299-305 Authors: Salgado J, Kalverda AP, Canters GW The relaxation enhancement caused by paramagnetic copper(II) is used to observe selectivelythe metal site of copper(I)-amicyanin by one- and two-dimensional NMR spectroscopy. Theparamagnetic effect is communicated to the diamagnetic protein through the...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] 1H-NMR study of a cobalt-substituted blue copper protein: Pseudomonas aeruginosa Co(I 1H-NMR study of a cobalt-substituted blue copper protein: Pseudomonas aeruginosa Co(II)-azurin. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H-NMR study of a cobalt-substituted blue copper protein: Pseudomonas aeruginosa Co(II)-azurin. Eur J Biochem. 1995 Jul 15;231(2):358-69 Authors: Salgado J, JimÃ©nez HR, Donaire A, Moratal JM Substitution of copper by cobalt in blue copper proteins gives a paramagnetic metalloderivative...	nmrlearner	Journal club	0	08-22-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off