De novo protein structure determination from near-atomic-resolution cryo-EM maps - Nature.com
De novo protein structure determination from near-atomic-resolution cryo-EM maps - Nature.com
De novo protein structure determination from near-atomic-resolution cryo-EM maps Nature.com
Despite these developments, little progress has been made in de novo model building into near-atomic-resolution cryo-EM density maps. Structural interpretation of cryo-EM maps typically starts with fitting an atomic X-ray or NMR structure into the map5.
[NMR paper] High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
Nat Commun. 2014;5:4976
Authors: Demers JP, Habenstein B, Loquet A, Kumar Vasa S, Giller K, Becker S, Baker D, Lange A, Sgourakis NG
Abstract
We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall...
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09-30-2014 02:18 PM
[NMR paper] Atomic Resolution Structure of a Protein Prepared by Non-Enzymatic His-Tag Removal. Crystallographic and NMR Study of GmSPI-2 Inhibitor.
Atomic Resolution Structure of a Protein Prepared by Non-Enzymatic His-Tag Removal. Crystallographic and NMR Study of GmSPI-2 Inhibitor.
Related Articles Atomic Resolution Structure of a Protein Prepared by Non-Enzymatic His-Tag Removal. Crystallographic and NMR Study of GmSPI-2 Inhibitor.
PLoS One. 2014;9(9):e106936
Authors: Kopera E, Bal W, Lenar?i? Živkovi? M, Dvornyk A, Kludkiewicz B, Grzelak K, Zhukov I, Zagórski-Ostoja W, Jaskolski M, Krzywda S
Abstract
Purification of suitable quantity of homogenous protein is very...
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09-19-2014 03:07 PM
[NMR paper] De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy.
De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy.
De Novo 3D Structure Determination from Sub-milligram Protein Samples by Solid-State 100 kHz MAS NMR Spectroscopy.
Angew Chem Int Ed Engl. 2014 Sep 15;
Authors: Agarwal V, Penzel S, Szekely K, Cadalbert R, Testori E, Oss A, Past J, Samoson A, Ernst M, Böckmann A, Meier BH
Abstract
Solid-state NMR spectroscopy is an emerging tool for structural studies of crystalline, membrane-associated, sedimented, and fibrillar...
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09-17-2014 11:54 AM
Huge Advance in Realizing Potential of cryo-EM for Atomic Resolution Imaging - AZoNano.com
<img alt="" height="1" width="1" />
Huge Advance in Realizing Potential of cryo-EM for Atomic Resolution Imaging
AZoNano.com
Most atomic resolution protein structures come from X-ray crystallography, a technique that is limited to samples which can be crystalized, a challenging task that can sometimes take years of effort, and from nuclear magnetic resonance (NMR ...
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Huge Advance in Realizing Potential of cryo-EM for Atomic Resolution Imaging - AZoNano.com
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[NMR paper] De novo protein structure determination using sparse NMR data.
De novo protein structure determination using sparse NMR data.
Related Articles De novo protein structure determination using sparse NMR data.
J Biomol NMR. 2000 Dec;18(4):311-8
Authors: Bowers PM, Strauss CE, Baker D
We describe a method for generating moderate to high-resolution protein structures using limited NMR data combined with the ab initio protein structure prediction method Rosetta. Peptide fragments are selected from proteins of known structure based on sequence similarity and consistency with chemical shift and NOE data. Models...
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[NMR paper] De novo determination of protein structure by NMR using orientational and long-range
De novo determination of protein structure by NMR using orientational and long-range order restraints.
Related Articles De novo determination of protein structure by NMR using orientational and long-range order restraints.
J Mol Biol. 2000 May 19;298(5):927-36
Authors: Hus JC, Marion D, Blackledge M
Orientational and novel long-range order restraints available from paramagnetic systems have been used to determine the backbone solution structure of the cytochrome c' protein to atomic resolution in the complete absence of restraints derived from...
De novo protein structure determination from near-atomic-resolution cryo-EM maps - Nature.com
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