[NMR paper] Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy.
Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy.
Related Articles Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy.
Methods Mol Biol. 2020;2141:233-245
Authors: Isaksson L, Pedersen A
Abstract
Cell-free protein synthesis (CFPS) is an established method to produce recombinant proteins and has been used in a wide variety of applications. The use of CFPS has almost from the onset been favorably linked to the production of isotopically...
nmrlearner
Journal club
0
07-23-2020 11:23 PM
[NMR paper] Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis.
Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis.
Related Articles Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis.
Prog Nucl Magn Reson Spectrosc. 2018 Apr;105:1-22
Authors: Hoffmann B, Löhr F, Laguerre A, Bernhard F, Dötsch V
Abstract
Preparation of a protein sample for liquid-state nuclear magnetic resonance (NMR) spectroscopy analysis requires optimization of many parameters. This review describes labeling strategies for obtaining assignments of...
nmrlearner
Journal club
0
03-20-2018 10:11 PM
[NMR paper] Sequential Protein Expression and Capsid Assembly In Cell: Towards the Study of Multi-Protein Viral Capsids Using Solid-State NMR Techniques.
Sequential Protein Expression and Capsid Assembly In Cell: Towards the Study of Multi-Protein Viral Capsids Using Solid-State NMR Techniques.
Related Articles Sequential Protein Expression and Capsid Assembly In Cell: Towards the Study of Multi-Protein Viral Capsids Using Solid-State NMR Techniques.
Biochemistry. 2018 Feb 21;:
Authors: Alphonse S, Itin B, Khayat R, Ghose R
Abstract
While solid-state NMR (ssNMR) has emerged as a powerful technique to study viral capsids, current studies are limited to capsids formed from single...
nmrlearner
Journal club
0
02-23-2018 03:44 AM
Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis
Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis
Publication date: Available online 7 December 2017
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Beate Hoffmann, Frank Löhr, Aisha Laguerre, Frank Bernhard, Volker Dötsch</br>
Preparation of a protein sample for liquid-state nuclear magnetic resonance (NMR) spectroscopy analysis requires optimization of many parameters. This review describes labeling strategies for obtaining assignments of protein resonances. Particular emphasis is placed on...
nmrlearner
Journal club
0
12-09-2017 07:49 AM
Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression
Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00488/20160721/images/medium/bi-2016-00488z_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00488
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/cYFGIK8-8JE
More...
nmrlearner
Journal club
0
07-22-2016 01:34 AM
Cell-free protein synthesis of perdeuterated proteins for NMR studies
Cell-free protein synthesis of perdeuterated proteins for NMR studies
Touraj Etezady-Esfarjani, Sebastian Hiller, Cristina Villalba and Kurt Wüthrich
Journal of Biomolecular NMR; 2007; 39(3); pp 229-238
Abstract:
Cell-free protein synthesis protocols for uniformly deuterated proteins typically yield low, non-uniform deuteration levels. This paper introduces an E. coli cell-extract, D-S30, which enables efficient production of proteins with high deuteration levels for all non-labile hydrogen atom positions. Potential applications of the new protocol may include production of proteins...
Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation - Frontiers
Linear Mode
