Chemists develop reversible method of tagging proteins - Eureka! Science News

		BioNMR > NMR community > Online News
Chemists develop reversible method of tagging proteins - Eureka! Science News

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-17-2012, 02:05 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,713

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Chemists develop reversible method of tagging proteins - Eureka! Science News

Chemists develop reversible method of tagging proteins - Eureka! Science News

Chemists develop reversible method of tagging proteins
Eureka! Science News
Subsequent reattachment of a fatty acid analogue reconstituted the protein complex to its natural state. By repeating the process again and again, while examining the molecular changes in the fatty acid with nuclear magnetic spectroscopy, or NMR ...

and more »
Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Large, medically important class of proteins starts to yield its secrets - Eureka! Science News Large, medically important class of proteins starts to yield its secrets - Eureka! Science News http://www.bionmr.com//nt0.ggpht.com/news/tbn/nP87yVy76N4T0M/6.jpg Eureka! Science News <img alt="" height="1" width="1" /> Large, medically important class of proteins starts to yield its secrets Eureka! Science News Such NMR structure studies were pioneered by WÃ¼thrichand one of the recent innovations has been the use of "microcoils" to enable NMR studies of minute quantities of precious protein samples in solution. WÃ¼thrich's lab, in collaboration with the ... and more »	nmrlearner	Online News	0	07-13-2012 09:15 AM
Researchers closer to understanding how proteins regulate immune system - Eureka! Science News Researchers closer to understanding how proteins regulate immune system - Eureka! Science News <img alt="" height="1" width="1" /> Researchers closer to understanding how proteins regulate immune system Eureka! Science News Using powerful magnets and a technique called nuclear magnetic resonance (NMR) spectroscopy, Gifford and Vogel determined the molecular structure of the interaction between the two proteins, providing important insight at the molecular level into how ... and more » Read here	nmrlearner	Online News	0	07-04-2012 01:08 AM
Researchers develop way to strengthen proteins with polymers - R & D Magazine Researchers develop way to strengthen proteins with polymers - R & D Magazine http://www.bionmr.com//nt1.ggpht.com/news/tbn/1e8ZLQCvJy_rwM/6.jpg Azom.com <img alt="" height="1" width="1" /> Researchers develop way to strengthen proteins with polymers R & D Magazine Proteins are widely used as drugsâ??insulin for diabetics is the best known ... next week of a new 0.75-mm solid state nuclear magnetic resonance (NMR) probe. Polymers Can be Used to Stabilize ProteinsAzom.com	nmrlearner	Online News	0	06-13-2012 11:34 AM
Alzheimer's protein structure suggests new treatment directions - Eureka! Science News http://www.bionmr.com//nt2.ggpht.com/news/tbn/Grqk4iT5yahTiM/6.jpg Eureka! Science News <img alt="" height="1" width="1" /> Alzheimer's protein structure suggests new treatment directions Eureka! Science News The Vanderbilt researchers used nuclear magnetic resonance and electron paragmagnetic resonance spectroscopy to determine the structure of C99, which has one membrane-spanning region. They were surprised to discover what appeared to be a "binding" ... and more » Alzheimer's protein structure suggests new treatment directions - Eureka! Science News	nmrlearner	Online News	0	06-01-2012 07:46 AM
New solid state NMR method can help visualize structure of proteins - News-Medical.net New solid state NMR method can help visualize structure of proteins - News-Medical.net <img alt="" height="1" width="1" /> New solid state NMR method can help visualize structure of proteins News-Medical.net For roughly a decade, a technique called solid state nuclear magnetic resonance (NMR) spectroscopy has allowed researchers to detect the arrangements of atoms in proteins that defy study by traditional laboratory tools such as X-ray crystallography. and more » Read here	nmrlearner	Online News	0	03-20-2012 10:37 AM
A new tool to reveal structure of proteins - Eureka! Science News A new tool to reveal structure of proteins - Eureka! Science News <img alt="" height="1" width="1" /> A new tool to reveal structure of proteins Eureka! Science News For roughly a decade, a technique called solid state nuclear magnetic resonance (NMR) spectroscopy has allowed researchers to detect the arrangements of atoms in proteins that defy study by traditional laboratory tools such as X-ray crystallography. and more » Read here	nmrlearner	Online News	0	03-19-2012 10:30 PM
Engineering [Ln(DPA)3]3â?? binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions Engineering 3â?? binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions Abstract Paramagnetic relaxation enhancements from unpaired electrons observed in nuclear magnetic resonance (NMR) spectra present powerful long-range distance restraints. The most frequently used paramagnetic tags, however, are tethered to the protein via disulfide bonds, requiring proteins with single cysteine residues for covalent attachment. Here we present a straightforward strategy to tag proteins site-specifically with paramagnetic lanthanides without a tether and...	nmrlearner	Journal club	0	07-26-2011 11:11 AM
A chaperone system guides tail-anchored membrane proteins to their destined ... - Eureka! Science News A chaperone system guides tail-anchored membrane proteins to their destined ... - Eureka! Science News http://nt3.ggpht.com/news/tbn/1-yHtxn6R10OfM/6.jpg Eureka! Science News <img alt="" height="1" width="1" /> A chaperone system guides tail-anchored membrane proteins to their destined ... Eureka! Science News ... methods such as X-Ray Crystallography and NMR-Spectroscopy as well as biochemical and cell-biological approaches. In detailed biophysical studies Volker DÃ¶tschÂ´s research group showed that the central cha-peron of the responsible protein complex, ... and more »	nmrlearner	Online News	0	07-24-2011 10:42 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off