[NMR paper] Tapping into the native Pseudomonas bacterial biofilm structure by high-resolution multidimensional solid-state NMR
Tapping into the native Pseudomonas bacterial biofilm structure by high-resolution multidimensional solid-state NMR
We present a multidimensional magic-angle spinning (MAS) solid-state NMR (ssNMR) study to characterize native Pseudomonas fluorescens colony biofilms at natural abundance without isotope-labelling. By using a high-resolution INEPT-based 2D ¹H-^(13)C ssNMR spectrum and thorough peak deconvolution at the 1D ssNMR spectra, approximately 80/134 (in 1D/2D) distinct biofilm chemical sites were identified. We compared CP and INEPT ^(13)C ssNMR spectra to differentiate signals...
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11-21-2023 03:55 PM
[NMR paper] Tapping into the native Pseudomonas Bacterial Biofilm Structure by High-Resolution 1D and 2D MAS solid-state NMR
Tapping into the native Pseudomonas Bacterial Biofilm Structure by High-Resolution 1D and 2D MAS solid-state NMR
We present a high-resolution 1D and 2D magic-angle spinning (MAS) solid-state NMR (ssNMR) study to characterize native Pseudomonas fluorescens colony biofilms at natural abundance without isotope-labelling. By using a high-resolution INEPT-based 2D ¹ H- ^(13) C ssNMR spectrum and thorough peak deconvolution approach at the 1D ssNMR spectra, approximately 80/134 (in 1D/2D) distinct biofilm chemical sites were identified. We compared CP and INEPT ^(13) C ssNMR spectra to different...
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10-24-2023 07:00 PM
[NMR paper] Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy.
Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy.
Related Articles Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy.
Nat Protoc. 2021 Jan 13;:
Authors: Narasimhan S, Pinto C, Lucini Paioni A, van der Zwan J, Folkers GE, Baldus M
Abstract
For a long time, solid-state nuclear magnetic resonance (ssNMR) has been employed to study complex biomolecular systems at the detailed chemical, structural, or dynamic level. Recent progress in...
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01-15-2021 04:25 PM
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy
Abstract
Membrane proteins play many critical roles in cells, mediating flow of material and information across cell membranes. They have evolved to perform these functions in the environment of a cell membrane, whose physicochemical properties are often different from those of common cell membrane mimetics used for structure determination. As a result, membrane proteins are difficult to study by traditional methods of structural biology, and they are significantly underrepresented in the protein structure...
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05-27-2015 10:39 AM
[NMR paper] Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.
Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.
Related Articles Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.
Protein Sci. 2015 May 13;
Authors: Brown LS, Ladizhansky V
Abstract
Membrane proteins play many critical roles in cells, mediating flow of material and information across cell membranes. They have evolved to perform these functions in the environment of a cell membrane, whose physicochemical properties are often different from those of common cell...
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05-15-2015 08:02 PM
[NMR paper] Structure Determination of Membrane Proteins in Their Native Phospholipid Bilayer Environment by Rotationally Aligned Solid-State NMR Spectroscopy.
Structure Determination of Membrane Proteins in Their Native Phospholipid Bilayer Environment by Rotationally Aligned Solid-State NMR Spectroscopy.
Structure Determination of Membrane Proteins in Their Native Phospholipid Bilayer Environment by Rotationally Aligned Solid-State NMR Spectroscopy.
Acc Chem Res. 2013 Jul 5;
Authors: Opella SJ
Abstract
One of the most important topics in experimental structural biology is determining the structures of membrane proteins. These structures represent one-third of all of the information...
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07-09-2013 02:47 PM
[NMR paper] Solid State NMR Strategy for Characterizing Native Membrane Protein Structures.
Solid State NMR Strategy for Characterizing Native Membrane Protein Structures.
Related Articles Solid State NMR Strategy for Characterizing Native Membrane Protein Structures.
Acc Chem Res. 2013 Mar 7;
Authors: Murray DT, Das N, Cross TA
Abstract
Unlike water soluble proteins, the structures of helicaltransmembrane proteins depend on a very complex environment. These proteins sit in the midst of dramatic electrical and chemical gradients and are often subject to variations in the lateral pressure profile, order parameters, dielectric...
Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy - Nature.com
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