[NMR paper] Multi-Dimensional Structure and Dynamics Landscape of Proteins in Mammalian Cells Revealed by In-Cell NMR
Multi-Dimensional Structure and Dynamics Landscape of Proteins in Mammalian Cells Revealed by In-Cell NMR
Governing function, half life and subcellular localization, the 3D structure and dynamics of proteins are in nature constantly changing in a tightly regulated manner to fulfill the physiological and adaptive requirements of the cells. To find evidence for this hypothesis, we applied in-cell NMR to three folded model proteins and propose that the splitting of cross peaks constitutes an atomic fingerprint of distinct structural states that arise from multiple target binding co-existing...
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11-17-2022 11:19 AM
[NMR paper] Visualizing Proteins in Mammalian Cells by 19F NMR spectroscopy
Visualizing Proteins in Mammalian Cells by 19F NMR spectroscopy
In-cell NMR spectroscopy is a powerful tool to investigate protein behavior in physiologically relevant environments. Although proven valuable for disordered proteins, we show that in commonly used 1 H- 15 N HSQC spectra of globular proteins, interactions with cellular components often broaden resonances beyond detection. This contrasts 19 F spectra in mammalian cells, in which signals are readily observed. Using several proteins, we demonstrate that surface charges and interaction with cellular...
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03-14-2022 04:46 AM
[NMR paper] In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
NMR has the resolution and specificity to determine atomic-level protein structures of isotopically labeled proteins in complex environments, and with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensitivity to detect proteins at their endogenous concentrations. However, DNP sensitivity enhancements are critically dependent on experimental conditions and sample composition. While some of these conditions are theoretically compatible with cellular viability,...
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11-02-2021 07:28 AM
[NMR paper] In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
Related Articles In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
Methods Mol Biol. 2020;2141:873-893
Authors: Gerez JA, Prymaczok NC, Riek R
Abstract
In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here we describe a detailed protocol to conduct in-cell NMR on the intrinsically...
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07-23-2020 11:23 PM
[NMR paper] Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Related Articles Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Methods Mol Biol. 2020;2141:857-871
Authors: Mate?ko-Burmann I, Burmann BM
Abstract
At the foundation of many cellular processes as well as a large number of diseases is the (mis)folding of important intrinsically disordered proteins (IDPs). Despite tremendous scientific efforts, the factors driving their structural changes within the cellular context remain poorly understood. In-cell NMR...
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07-23-2020 11:23 PM
[NMR paper] Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Nat Protoc. 2016 Jun;11(6):1101-1111
Authors: Barbieri L, Luchinat E, Banci L
Abstract
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells - Nature.com
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