In-cell protein stability promotes antimicrobial resistance of metallo-? ... - Nature.com

		BioNMR > NMR community > Online News
In-cell protein stability promotes antimicrobial resistance of metallo-? ... - Nature.com

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-15-2023, 09:27 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

In-cell protein stability promotes antimicrobial resistance of metallo-? ... - Nature.com

In-cell protein stability promotes antimicrobial resistance of metallo-? ... - Nature.com

In-cell protein stability promotes antimicrobial resistance of metallo-? ... Nature.com Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide Dynamic nuclear polarization NMR spectroscopy was used to investigate the effect of the antimicrobial peptide (AMP) maculatin 1.1 on E. coli cells. The enhanced ^(15)N NMR signals from nucleic acids, proteins and lipids identified a number of unanticipated physiological responses to peptide stress, revealing that membrane-active AMPs can have a multi-target impact on E. coli cells. DNP-enhanced ^(15)N-observed ^(31)P-dephased REDOR NMR allowed monitoring how Mac1 induced DNA condensation and... More...	nmrlearner	Journal club	0	09-26-2022 02:08 AM
New diterpenes from the marine sponge Spongionella sp. overcome drug resistance in prostate cancer by inhibition of P-glycoprotein \| Scientific Reports - Nature.com New diterpenes from the marine sponge Spongionella sp. overcome drug resistance in prostate cancer by inhibition of P-glycoprotein \| Scientific Reports - Nature.com New diterpenes from the marine sponge Spongionella sp. overcome drug resistance in prostate cancer by inhibition of P-glycoprotein \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	08-09-2022 01:50 PM
Spatial organization of hydrophobic and charged residues affects protein thermal stability and binding affinity \| Scientific Reports - Nature.com Spatial organization of hydrophobic and charged residues affects protein thermal stability and binding affinity \| Scientific Reports - Nature.com Spatial organization of hydrophobic and charged residues affects protein thermal stability and binding affinity \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	07-18-2022 03:28 PM
[ASAP] Designed Trp-Cage Proteins with Antimicrobial Activity and Enhanced Stability Designed Trp-Cage Proteins with Antimicrobial Activity and Enhanced Stability https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.1c00567/20211006/images/medium/bi1c00567_0006.gif Biochemistry DOI: 10.1021/acs.biochem.1c00567 http://feeds.feedburner.com/~r/acs/bichaw/~4/2fzm9ONNk-4 More...	nmrlearner	Journal club	0	10-07-2021 04:35 PM
[NMR paper] Insights into protein stability in cell lysate by 19F NMR spectroscopy. Insights into protein stability in cell lysate by 19F NMR spectroscopy. Related Articles Insights into protein stability in cell lysate by 19F NMR spectroscopy. Chembiochem. 2020 Aug 12;: Authors: Welte H, Kovermann M Abstract In living organisms, protein folding and function take place in an inhomogeneous, highly crowded environment possessing a concentration of diverse macromolecules of up to 400*g/L. It has been shown that the intracellular environment has a pronounced effect on the stability, dynamics and function of the...	nmrlearner	Journal club	0	08-15-2020 05:51 AM
Proteinâ??protein interaction inhibitor tackles drug resistance - Nature.com Proteinâ??protein interaction inhibitor tackles drug resistance - Nature.com http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcRJ1m0HrLxCJOVezkUwdp6bTbUk8qIf1ZXpjDbBKz9C9UvAZb_uCsCdDef4-1soEeLC0rNV-OM Nature.com <img alt="" height="1" width="1"> Proteinâ??protein interaction inhibitor tackles drug resistance Nature.com NMR spectroscopy was then used to determine the mechanism of action of the most potent compound, termed iKIX1. The high-resolution structure showed that iKIX1 binds to the three-helix hydrophobic groove of the KIX domain of Gal11A, engaging the same ...	nmrlearner	Online News	0	04-01-2016 09:13 AM
19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes fro 19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes from bacterial protoplasts and human erythrocytes. Related Articles 19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes from bacterial protoplasts and human erythrocytes. J Am Chem Soc. 2010 Jul 7;132(26):8822-4 Authors: Ieronimo M, Afonin S, Koch K, Berditsch M, Wadhwani P, Ulrich AS (19)F NMR is a unique tool to examine the structure of fluorine-labeled peptides in their native cellular environment, due to an exquisite sensitivity...	nmrlearner	Journal club	0	10-05-2010 12:11 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off