Biophysicists propose new approach for membrane protein crystallization - EurekAlert (press release)
Biophysicists propose new approach for membrane protein crystallization - EurekAlert (press release)
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EurekAlert (press release)
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Biophysicists propose new approach for membrane protein crystallization
EurekAlert (press release)
Nanodiscs are also widely used to study the structure of membrane proteins using nuclear magnetic resonance (NMR), cryo-electron microscopy, atomic force microscopy, and other methods. Direct use of...
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03-07-2017 08:21 AM
Light microscopy provides a deep look into protein structure - Phys.org - Phys.Org
Light microscopy provides a deep look into protein structure - Phys.org - Phys.Org
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Phys.Org
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Light microscopy provides a deep look into protein structure - Phys.org
Phys.Org
Light microscopy continues to reveal the microscopic world at an ever increasing resolution. Using a new method coined COLD, scientists at the Max Planck ...
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01-25-2017 11:13 PM
Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach
Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach
Abstract
The β-barrel assembly machinery (BAM) is involved in folding and insertion of outer membrane proteins in Gram-negative bacteria, a process that is still poorly understood. With its 790 residues, BamA presents a challenge to current NMR methods. We utilized a â??divide and conquerâ?? approach in which we first obtained resonance assignments for BamAâ??s periplasmic POTRA domains 4 and 5 by solution NMR. Comparison of...
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01-08-2015 01:02 AM
[NMR paper] Structure and Topology of the Huntingtin 1-17 Membrane Anchor by*a*Combined Solution and Solid-State NMR Approach.
Structure and Topology of the Huntingtin 1-17 Membrane Anchor by*a*Combined Solution and Solid-State NMR Approach.
Structure and Topology of the Huntingtin 1-17 Membrane Anchor by*a*Combined Solution and Solid-State NMR Approach.
Biophys J. 2013 Aug 6;105(3):699-710
Authors: Michalek M, Salnikov ES, Bechinger B
Abstract
The very amino-terminal domain of the huntingtin protein is directly located upstream of the protein's polyglutamine tract, plays a decisive role in several important properties of this large protein and in the development...
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08-13-2013 04:26 PM
An ensemble dynamics approach to decipher solid-state NMR observables of membrane proteins
An ensemble dynamics approach to decipher solid-state NMR observables of membrane proteins
February 2012
Publication year: 2012
Source:Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1818, Issue 2</br>
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Solid-state NMR (SSNMR) is an invaluable tool for determining orientations of membrane proteins and peptides in lipid bilayers. Such orientational descriptions provide essential information about membrane protein functions. However, when a semi-static single conformer model is used to interpret various SSNMR observables, important dynamics information can...
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02-03-2013 10:13 AM
An ensemble dynamics approach to decipher solid-sate NMR observables of membrane proteins.
An ensemble dynamics approach to decipher solid-sate NMR observables of membrane proteins.
An ensemble dynamics approach to decipher solid-sate NMR observables of membrane proteins.
Biochim Biophys Acta. 2011 Aug 8;
Authors: Im W, Jo S, Kim T
Abstract
Solid-state NMR (SSNMR) is an invaluable tool for determining orientations of membrane proteins and peptides in lipid bilayers. Such orientational descriptions provide essential information about membrane protein functions. However, when a semi-static single conformer model is used to...