[NMR paper] Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning
Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning
The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent...
nmrlearner
Journal club
0
06-02-2021 05:10 PM
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
Abstract
Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE...
nmrlearner
Journal club
0
04-25-2020 12:56 PM
[NMR paper] Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.
Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.
Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR.
J Biomol NMR. 2018 Mar 03;:
Authors: Tolchard J, Pandey MK, Berbon M, Noubhani A, Saupe SJ, Nishiyama Y, Habenstein B, Loquet A
Abstract
We present a new solid-state NMR proton-detected three-dimensional experiment dedicated to the observation of protein proton...
nmrlearner
Journal club
0
03-05-2018 01:04 PM
Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR
Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR
Abstract
We present a new solid-state NMR proton-detected three-dimensional experiment dedicated to the observation of protein proton side chain resonances in nano-liter volumes. The experiment takes advantage of very fast magic angle spinning and double quantum 13Cā??13C transfer to establish efficient (H)CCH correlations detected on side chain protons. Our approach is demonstrated on the HET-s prion domain in its...
nmrlearner
Journal club
0
03-03-2018 12:01 PM
[NMR paper] Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Related Articles Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Acc Chem Res. 2017 Mar 29;:
Authors: Zhang R, Mroue KH, Ramamoorthy A
Abstract
Protons are vastly abundant in a wide range of exciting macromolecules and thus can be a powerful probe to investigate the structure and dynamics at atomic resolution using solid-state NMR (ssNMR) spectroscopy. Unfortunately, the high signal sensitivity, afforded by the high...
nmrlearner
Journal club
0
03-30-2017 06:42 PM
[NMR paper] Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Related Articles Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Nat Protoc. 2017 Apr;12(4):764-782
Authors: Fricke P, Chevelkov V, Zinke M, Giller K, Becker S, Lange A
Abstract
Solid-state NMR (ssNMR) is a technique that allows the study of protein structure and dynamics at atomic detail. In contrast to X-ray crystallography and...
nmrlearner
Journal club
0
03-10-2017 04:23 PM
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
From The DNP-NMR Blog:
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
A large portion of the magnetization in a CP experiment remains unused after an experiment and different strategies exist to make better use of the proton magnetization. Here the authors show their results of testing 7 different cp schemes. Although not directly related to DNP these techniques are still very valuable to increase the sensitivity of an NMR experiment especially in combination with DNP.
Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning - Nature.com
Linear Mode
