Auto-induction medium containing glyphosate for high-level incorporation of ... - Bio

		BioNMR > NMR community > Online News
Auto-induction medium containing glyphosate for high-level incorporation of ... - Bio

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-16-2010, 03:50 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,700

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Auto-induction medium containing glyphosate for high-level incorporation of ... - Bio

Auto-induction medium containing glyphosate for high-level incorporation of ... - BioTechniques.com

Auto-induction medium containing glyphosate for high-level incorporation of ...
BioTechniques.com
Quantitation of protein expression in a cell-free system: efficient detection of yields and 19 F NMR to identify folded protein. J. Biomol. NMR 31:11-19. 7. ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Ultra High-Resolution NMR: Sustained Induction Decays of Long-Lived Coherences Ultra High-Resolution NMR: Sustained Induction Decays of Long-Lived Coherences Aure?lien Bornet, Sami Jannin, J. A. (Ton) Konter, Patrick Hautle, Ben van den Brandt and Geoffrey Bodenhausen http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2052792/aop/images/medium/ja-2011-052792_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja2052792 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/F3VMiSJp4h4	nmrlearner	Journal club	0	09-02-2011 07:31 PM
[NMR paper] Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled prote Auto-induction medium for the production of - and -labeled proteins for NMR screening and structure determination. Related Articles Auto-induction medium for the production of - and -labeled proteins for NMR screening and structure determination. Protein Expr Purif. 2005 Apr;40(2):268-78 Authors: Tyler RC, Sreenath HK, Singh S, Aceti DJ, Bingman CA, Markley JL, Fox BG Protocols have been developed and applied for the high-throughput production of - or -labeled proteins using the conditional methionine auxotroph Escherichia coli B834. The...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] Design, high-level expression, purification and characterization of soluble fragments Design, high-level expression, purification and characterization of soluble fragments of the hepatitis C virus NS3 RNA helicase suitable for NMR-based drug discovery methods and mechanistic studies. Related Articles Design, high-level expression, purification and characterization of soluble fragments of the hepatitis C virus NS3 RNA helicase suitable for NMR-based drug discovery methods and mechanistic studies. Protein Eng. 2001 Aug;14(8):573-82 Authors: Gesell JJ, Liu D, Madison VS, Hesson T, Wang YS, Weber PC, Wyss DF RNA helicases represent...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] High-level bacterial expression and 15N-alanine-labeling of bovine trypsin. Applicati High-level bacterial expression and 15N-alanine-labeling of bovine trypsin. Application to the study of trypsin-inhibitor complexes and trypsinogen activation by NMR spectroscopy. Related Articles High-level bacterial expression and 15N-alanine-labeling of bovine trypsin. Application to the study of trypsin-inhibitor complexes and trypsinogen activation by NMR spectroscopy. Biochemistry. 2001 May 29;40(21):6275-83 Authors: Peterson FC, Gordon NC, Gettins PG We describe here the high-level expression of bovine trypsinogen in E. coli, its...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] High-level 2H/13C/15N labeling of proteins for NMR studies. High-level 2H/13C/15N labeling of proteins for NMR studies. Related Articles High-level 2H/13C/15N labeling of proteins for NMR studies. J Biomol NMR. 1995 Jun;5(4):339-44 Authors: Venters RA, Huang CC, Farmer BT, Trolard R, Spicer LD, Fierke CA The protein human carbonic anhydrase II (HCA II) has been isotopically labeled with 2H, 13C and 15N for high-resolution NMR assignment studies and pulse sequence development. To increase the sensitivity of several key 1H/13C/15N triple-resonance correlation experiments, 2H has been incorporated into...	nmrlearner	Journal club	0	08-22-2010 03:41 AM
[NMR paper] High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and id High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements. FEBS Lett. 1999 Apr 1;448(1):33-7 Authors: Mine S, Ueda T, Hashimoto Y, Tanaka Y,...	nmrlearner	Journal club	0	08-21-2010 04:03 PM
Auto-induction appendix - LsbWiki Auto-induction appendix - LsbWiki More...	nmrlearner	NMR bookmarks	0	08-19-2010 02:34 PM
Introductory NMR & MRI: Video 04: Acquiring a Free Induction Decay (FID) Introductory NMR & MRI: Video 04: Acquiring a Free Induction Decay (FID) http://i.ytimg.com/vi/MPXbDDRumwM/default.jpg Introductory NMR & MRI: Video 04: Acquiring a Free Induction Decay (FID) Paul Callaghan gives an introduction to NMR and MRI. This is the 4th video of the series in which the acquisition of an FID is explained. 10 episode series produced by Magritek Ltd. From: magritek Views: 7742 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif...	nmrlearner	NMR educational videos	0	08-18-2010 01:38 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off