[NMR paper] Solution-state NMR assignment and secondary structure analysis of the monomeric Pseudomonas biofilm-forming functional amyloid accessory protein FapA
Solution-state NMR assignment and secondary structure analysis of the monomeric Pseudomonas biofilm-forming functional amyloid accessory protein FapA
FapA is an accessory protein within the biofilm forming functional bacterial amyloid related fap-operon in Pseudomonas, and maybe a chaperone for FapC controlling its fibrillization. To allow further structural analysis, here we present a complete sequential assignment of ¹H(amide), ^(13)C(?), ^(13)C(?), and ^(15)N NMR resonances for the functional form of the monomeric soluble FapA protein, comprising amino acids between 29 and 152. From...
nmrlearner
Journal club
0
10-06-2023 10:37 PM
[NMR paper] Quantification of protein secondary structure by (13)C solid-state NMR.
Quantification of protein secondary structure by (13)C solid-state NMR.
Related Articles Quantification of protein secondary structure by (13)C solid-state NMR.
Anal Bioanal Chem. 2016 Apr 11;
Authors: Andrade FD, Forato LA, Bernardes Filho R, Colnago LA
Abstract
High-resolution (13)C solid-state NMR stands out as one of the most promising techniques to solve the structure of insoluble proteins featuring biological and technological importance. The simplest nuclear magnetic resonance (NMR) spectroscopy method to quantify the...
nmrlearner
Journal club
0
04-14-2016 12:01 PM
[NMR paper] Solid-state NMR Study of the YadA Membrane-Anchor Domain in the Bacterial Outer Membrane.
Solid-state NMR Study of the YadA Membrane-Anchor Domain in the Bacterial Outer Membrane.
Related Articles Solid-state NMR Study of the YadA Membrane-Anchor Domain in the Bacterial Outer Membrane.
Angew Chem Int Ed Engl. 2015 Aug 31;
Authors: Shahid SA, Nagaraj M, Chauhan N, Franks TW, Bardiaux B, Habeck M, Orwick-Rydmark M, Linke D, van Rossum BJ
Abstract
MAS-NMR was used to study the structure and dynamics at ambient temperatures of the membrane-anchor domain of YadA (YadA-M) in a pellet of the outer membrane of E. coli in...
nmrlearner
Journal club
0
09-04-2015 12:45 PM
[NMR paper] Practical use of chemical shift databases for protein solid-state NMR: 2D chemical shift maps and amino-acid assignment with secondary-structure information.
Practical use of chemical shift databases for protein solid-state NMR: 2D chemical shift maps and amino-acid assignment with secondary-structure information.
Practical use of chemical shift databases for protein solid-state NMR: 2D chemical shift maps and amino-acid assignment with secondary-structure information.
J Biomol NMR. 2013 Apr 28;
Authors: Fritzsching KJ, Yang Y, Schmidt-Rohr K, Hong M
Abstract
We introduce a Python-based program that utilizes the large database of (13)C and (15)N chemical shifts in the Biological Magnetic...
Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR - Nature.com
Linear Mode
