Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR

		BioNMR > NMR community > Online News
Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR \| Scientific Reports - Nature.com

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-28-2023, 10:50 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR | Scientific Reports - Nature.com

Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR | Scientific Reports - Nature.com

Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR | Scientific Reports Nature.com Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
The near-symmetry of protein oligomers: NMR-derived structures \| Scientific Reports - Nature.com The near-symmetry of protein oligomers: NMR-derived structures \| Scientific Reports - Nature.com The near-symmetry of protein oligomers: NMR-derived structures \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	12-28-2023 10:50 PM
Retrospective study for the universal applicability of the residue-based linear free energy relationship in the two-state exchange of protein molecules \| Scientific Reports - Nature.com Retrospective study for the universal applicability of the residue-based linear free energy relationship in the two-state exchange of protein molecules \| Scientific Reports - Nature.com Retrospective study for the universal applicability of the residue-based linear free energy relationship in the two-state exchange of protein molecules \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	10-08-2022 07:03 AM
AlphaFold2 models indicate that protein sequence determines both structure and dynamics \| Scientific Reports - Nature.com AlphaFold2 models indicate that protein sequence determines both structure and dynamics \| Scientific Reports - Nature.com AlphaFold2 models indicate that protein sequence determines both structure and dynamics \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	08-11-2022 01:49 PM
Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble \| Scientific Reports - Nature.com Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble \| Scientific Reports - Nature.com Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	08-11-2022 01:49 PM
Role of a bacterial glycolipid in Sec-independent membrane protein insertion \| Scientific Reports - Nature.com Role of a bacterial glycolipid in Sec-independent membrane protein insertion \| Scientific Reports - Nature.com Role of a bacterial glycolipid in Sec-independent membrane protein insertion \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	07-18-2022 03:28 PM
Crystal structure of potato 14-3-3 protein St14f revealed the importance of helix I in StFDL1 recognition \| Scientific Reports - Nature.com Crystal structure of potato 14-3-3 protein St14f revealed the importance of helix I in StFDL1 recognition \| Scientific Reports - Nature.com Crystal structure of potato 14-3-3 protein St14f revealed the importance of helix I in StFDL1 recognition \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	07-09-2022 07:39 AM
Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori \| Scientific Reports - Nature.com Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori \| Scientific Reports - Nature.com Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori \| Scientific Reports Nature.com Read here	nmrlearner	Online News	0	12-31-2021 08:07 AM
[NMR paper] Solid-state NMR Study of the YadA Membrane-Anchor Domain in the Bacterial Outer Membrane. Solid-state NMR Study of the YadA Membrane-Anchor Domain in the Bacterial Outer Membrane. Related Articles Solid-state NMR Study of the YadA Membrane-Anchor Domain in the Bacterial Outer Membrane. Angew Chem Int Ed Engl. 2015 Aug 31; Authors: Shahid SA, Nagaraj M, Chauhan N, Franks TW, Bardiaux B, Habeck M, Orwick-Rydmark M, Linke D, van Rossum BJ Abstract MAS-NMR was used to study the structure and dynamics at ambient temperatures of the membrane-anchor domain of YadA (YadA-M) in a pellet of the outer membrane of E. coli in...	nmrlearner	Journal club	0	09-04-2015 12:45 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off