Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience

		BioNMR > NMR community > Online News
Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-28-2017, 10:21 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience

Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience

Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies
SelectScience
Perdeuteration of polypeptides is a useful technique for studying large polypeptides in solution, however this can be difficult to do in vivo. This application note summarizes work using cell-free synthesis methods employing both H2O-based and 2H2O ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience <img alt="" height="1" width="1"> In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies SelectScience Perdeuteration of polypeptides is a useful technique for studying large polypeptides in solution, however this can be difficult to do in vivo. This application note summarizes work using cell-free synthesis methods employing both H2O-based and 2H2O ... Read here	nmrlearner	Online News	0	07-28-2017 08:34 AM
Application Note: Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using ... - SelectScience Application Note: Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using ... - SelectScience <img alt="" height="1" width="1"> Application Note: Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using ... SelectScience Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their interaction with ligands of pharmaceutical interest. To be useful for most important NMR studies, a labeled protein has to be expressed with baculovirus-infected ... and more » Read here	nmrlearner	Online News	0	07-23-2017 11:38 AM
Asynchronous through-bond homonuclear isotropic mixing: application to carbonâ??carbon transfer in perdeuterated proteins under MAS Asynchronous through-bond homonuclear isotropic mixing: application to carbonâ??carbon transfer in perdeuterated proteins under MAS Abstract Multiple-bond carbonâ??carbon homonuclear mixing is a hurdle in extensively deuterated proteins and under fast MAS due to the absence of an effective proton dipolar-coupling network. Such conditions are now commonly employed in solid-state NMR spectroscopy. Here, we introduce an isotropic homonuclear 13Câ??13C through-bond mixing sequence, MOCCA, for the solid state. Even though applied under MAS, this scheme...	nmrlearner	Journal club	0	08-29-2015 09:18 PM
Erratum to: Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O Erratum to: Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O Erratum to: Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O Content Type Journal Article Category Erratum Pages 1-1 DOI 10.1007/s10858-011-9562-9 Authors	nmrlearner	Journal club	0	09-20-2011 05:02 AM
Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O Abstract Selectively isotope labelled protein samples can be prepared in vivo or in vitro from selectively labelled amino acids but, in many cases, metabolic conversions between different amino acids result in isotope scrambling. The best results are obtained by cell-free protein synthesis, where metabolic enzymes are generally less active, but isotope scrambling can never be suppressed completely. We show that...	nmrlearner	Journal club	0	02-16-2011 09:34 PM
[NMR paper] Synthesis of region-labelled proteins for NMR studies by in vitro translation of colu Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs. Biochimie. 1997 Jul;79(7):415-22 Authors: Pavlov MY, Freistroffer DV, Ehrenberg M A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Synthesis of region-labelled proteins for NMR studies by in vitro translation of colu Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs. Biochimie. 1997 Jul;79(7):415-22 Authors: Pavlov MY, Freistroffer DV, Ehrenberg M A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
Cell-free protein synthesis of perdeuterated proteins for NMR studies Cell-free protein synthesis of perdeuterated proteins for NMR studies Touraj Etezady-Esfarjani, Sebastian Hiller, Cristina Villalba and Kurt Wüthrich Journal of Biomolecular NMR; 2007; 39(3); pp 229-238 Abstract: Cell-free protein synthesis protocols for uniformly deuterated proteins typically yield low, non-uniform deuteration levels. This paper introduces an E. coli cell-extract, D-S30, which enables efficient production of proteins with high deuteration levels for all non-labile hydrogen atom positions. Potential applications of the new protocol may include production of proteins...	Deano	Journal club	0	08-14-2008 10:01 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off