Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... - SelectScience

		BioNMR > NMR community > Online News
Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... - SelectScience

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-01-2017, 12:04 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,778

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... - SelectScience

Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... - SelectScience

Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ...
SelectScience
Solid-state magic-angle spinning (MAS)-NMR has rapidly been developing into a structure determination technique for biological macromolecules, as it enables the study of membrane proteins in their native lipid environment, as well as the study of ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... - SelectScience Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... - SelectScience <img alt="" height="1" width="1"> Application Note: Improving Signal Intensities and Assignment of Membrane Proteins by Solid-State MAS-NMR Using ... SelectScience Solid-state magic-angle spinning (MAS)-NMR has rapidly been developing into a structure determination technique for biological macromolecules, as it enables the study of membrane proteins in their native lipid environment, as well as the study of ... Read here	nmrlearner	Online News	0	07-31-2017 11:54 AM
Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies - SelectScience <img alt="" height="1" width="1"> Application Note: In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies SelectScience Perdeuteration of polypeptides is a useful technique for studying large polypeptides in solution, however this can be difficult to do in vivo. This application note summarizes work using cell-free synthesis methods employing both H2O-based and 2H2O ... Read here	nmrlearner	Online News	0	07-28-2017 10:21 PM
Application Note: Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using ... - SelectScience Application Note: Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using ... - SelectScience <img alt="" height="1" width="1"> Application Note: Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using ... SelectScience Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their interaction with ligands of pharmaceutical interest. To be useful for most important NMR studies, a labeled protein has to be expressed with baculovirus-infected ... and more » Read here	nmrlearner	Online News	0	07-23-2017 11:38 AM
[NMR paper] Proton detection for signal enhancement in solid-state NMR experiments on mobile species in membrane proteins. Proton detection for signal enhancement in solid-state NMR experiments on mobile species in membrane proteins. Related Articles Proton detection for signal enhancement in solid-state NMR experiments on mobile species in membrane proteins. J Biomol NMR. 2015 Oct 22; Authors: Ward ME, Ritz E, Ahmed MA, Bamm VV, Harauz G, Brown LS, Ladizhansky V Abstract Direct proton detection is becoming an increasingly popular method for enhancing sensitivity in solid-state nuclear magnetic resonance spectroscopy. Generally, these experiments...	nmrlearner	Journal club	0	10-24-2015 06:43 PM
Proton detection for signal enhancement in solid-state NMR experiments on mobile species in membrane proteins Proton detection for signal enhancement in solid-state NMR experiments on mobile species in membrane proteins Abstract Direct proton detection is becoming an increasingly popular method for enhancing sensitivity in solid-state nuclear magnetic resonance spectroscopy. Generally, these experiments require extensive deuteration of the protein, fast magic angle spinning (MAS), or a combination of both. Here, we implement direct proton detection to selectively observe the mobile entities in fully-protonated membrane proteins at moderate MAS frequencies. We...	nmrlearner	Journal club	0	10-23-2015 02:14 PM
[NMR paper] Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers. Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers. Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers. J Magn Reson. 2014 Mar 1;242C:224-232 Authors: Das BB, Zhang H, Opella SJ Abstract A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of heteronuclear dipolar coupling...	nmrlearner	Journal club	0	04-06-2014 02:01 AM
[NMR paper] Sensitivity and resolution enhancement of oriented solid-state NMR: Application to membrane proteins. Sensitivity and resolution enhancement of oriented solid-state NMR: Application to membrane proteins. Related Articles Sensitivity and resolution enhancement of oriented solid-state NMR: Application to membrane proteins. Prog Nucl Magn Reson Spectrosc. 2013 Nov;75:50-68 Authors: Gopinath T, Mote KR, Veglia G Abstract Oriented solid-state NMR (O-ssNMR) spectroscopy is a major technique for the high-resolution analysis of the structure and topology of transmembrane proteins in native-like environments. Unlike magic angle spinning (MAS)...	nmrlearner	Journal club	0	10-29-2013 08:21 PM
Sensitivity and Resolution Enhancement of Oriented Solid-State NMR: Application to Membrane Proteins Sensitivity and Resolution Enhancement of Oriented Solid-State NMR: Application to Membrane Proteins Publication date: Available online 12 August 2013 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> Author(s): T. Gopinath , Kaustubh R. Mote , Gianluigi Veglia</br> Oriented solid-state NMR (O-ssNMR) spectroscopy is a major technique for the high-resolution analysis of the structure and topology of transmembrane proteins in native-like environments. Unlike magic angle spinning (MAS) techniques, O-ssNMR spectroscopy requires membrane protein...	nmrlearner	Journal club	0	08-13-2013 04:09 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off