Alternative ingredients to be used in poultry feed - All about feed
Alternative ingredients to be used in poultry feed All about feed
The in vitro digestibility of DM, starch and crude protein were determined according to the method of Babinszky (1990) and the structure of grain was assessed using electron microscopy and nuclear magnetic resonance techniques. The scanning electron ...
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[NMR paper] Low resolution (1)H NMR assignment of proton populations in pound cake and its polymeric ingredients.
Low resolution (1)H NMR assignment of proton populations in pound cake and its polymeric ingredients.
Related Articles Low resolution (1)H NMR assignment of proton populations in pound cake and its polymeric ingredients.
Food Chem. 2013 Aug 15;139(1-4):120-8
Authors: Luyts A, Wilderjans E, Waterschoot J, Van Haesendonck I, Brijs K, Courtin CM, Hills B, Delcour JA
Abstract
Based on a model system approach, five different proton populations were distinguished in pound cake crumb using one dimensional low resolution (1)H NMR spectroscopy. In...
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04-09-2013 06:31 PM
Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy
Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy
Abstract Longitudinal exchange experiments facilitate the quantification of the rates of interconversion between the exchanging species, along with their longitudinal relaxation rates, by analyzing the time-dependence of direct correlation and exchange cross peaks. Here we present a simple and robust alternative to this strategy, which is based on the combination of two complementary experiments, one with and one without resolving exchange cross peaks. We show that...
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09-30-2011 08:01 PM
Alternative SAIL-Trp for robust aromatic signal assignment and determination of the Ï?2 conformation by intra-residue NOEs
Alternative SAIL-Trp for robust aromatic signal assignment and determination of the Ï?2 conformation by intra-residue NOEs
Abstract Tryptophan (Trp) residues are frequently found in the hydrophobic cores of proteins, and therefore, their side-chain conformations, especially the precise locations of the bulky indole rings, are critical for determining structures by NMR. However, when analyzing -proteins, the observation and assignment of the ring signals are often hampered by excessive overlaps and tight spin couplings. These difficulties have been greatly alleviated by using...
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09-27-2011 07:04 AM
[MWClarkson blog] Alternative side-chain structures from methyl CPMG
Alternative side-chain structures from methyl CPMG
http://www.researchblogging.org/public/citation_icons/rb2_large_gray.pngAs I have mentioned before on this blog, the use of tools like CS-ROSETTA holds the promise of determining protein structures using only the chemical shifts of its backbone atoms. In addition to potentially making NOEs and RDCs redundant, this technology allows biologists to determine the conformations of minor members of the structural ensemble, which are very difficult to obtain using conventional approaches in population-dominated techniques like NMR and X-ray...
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06-21-2011 03:31 AM
Application of SAIL phenylalanine and tyrosine with alternative isotope-labeling patterns for protein structure determination
Application of SAIL phenylalanine and tyrosine with alternative isotope-labeling patterns for protein structure determination
Abstract The extensive collection of NOE constraint data involving the aromatic ring signals is essential for accurate protein structure determination, although it is often hampered in practice by the pervasive signal overlapping and tight spin couplings for aromatic rings. We have prepared various types of stereo-array isotope labeled phenylalanines (ε- and ζ-SAIL Phe) and tyrosine (ε-SAIL Tyr) to overcome these problems (Torizawa et al. 2005), and proven...
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[NMR paper] 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative mea
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Related Articles 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes.
NMR Biomed. 1994 Nov;7(7):330-8
Authors: Gilboa H, Chapman BE, Kuchel PW
The 19F NMR Ca(2+)-indicator molecule 5,5'-difluoro-1,2-bis(o-...
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08-22-2010 03:29 AM
[U. of Ottawa NMR Facility Blog] Temperature Calibration - An Alternative Method
Temperature Calibration - An Alternative Method
It is well known that the actual temperature of a sample in an NMR probe is not necessarily the same as that read from the variable temperature unit on the spectrometer. This is because the thermocouple used by the variable temperature unit is below the sample tube and not in the center of the rf coil where the NMR measurements are made. One normally must make a calibration plot for the actual temperature vs. the set temperature. For temperatures above room temperature this can be done by employing the known temperature dependent chemical...