Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR | Communications Biology - Nature.com

		BioNMR > NMR community > Online News
Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR \| Communications Biology - Nature.com

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-24-2024, 08:25 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR | Communications Biology - Nature.com

Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR | Communications Biology - Nature.com

Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR | Communications Biology Nature.com Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression \| Communications Biology - Nature.com Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression \| Communications Biology - Nature.com Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression \| Communications Biology Nature.com Read here	nmrlearner	Online News	0	07-24-2024 08:25 AM
Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR \| Communications Biology - Nature.com Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR \| Communications Biology - Nature.com Active metabolism unmasks functional protein–protein interactions in real time in-cell NMR \| Communications Biology Nature.com Read here	nmrlearner	Online News	0	12-28-2023 10:50 PM
Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression \| Communications Biology - Nature.com Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression \| Communications Biology - Nature.com Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression \| Communications Biology Nature.com Read here	nmrlearner	Online News	0	12-03-2022 10:25 PM
[NMR paper] Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell...	nmrlearner	Journal club	0	03-30-2021 08:29 AM
[NMR paper] Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR. Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR. Related Articles Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR. Commun Biol. 2020 May 21;3(1):249 Authors: Breindel L, Burz DS, Shekhtman A Abstract Protein-protein interactions, PPIs, underlie most cellular processes, but many PPIs depend on a particular metabolic state that can only be observed in live, actively metabolizing cells. Real time in-cell NMR spectroscopy, RT-NMR, utilizes a...	nmrlearner	Journal club	0	05-24-2020 05:50 AM
Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-TargetedAntibiotics Modulate Quinary Protein Interactions Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-TargetedAntibiotics Modulate Quinary Protein Interactions http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00938/20180108/images/medium/bi-2017-00938t_0004.gif Biochemistry DOI: 10.1021/acs.biochem.7b00938 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/ylGRHlv1kYM More...	nmrlearner	Journal club	0	01-09-2018 09:41 AM
[NMR paper] Real Time In-cell NMR: Ribosome Targeted Antibiotics Modulate Quinary Protein Interactions. Real Time In-cell NMR: Ribosome Targeted Antibiotics Modulate Quinary Protein Interactions. Real Time In-cell NMR: Ribosome Targeted Antibiotics Modulate Quinary Protein Interactions. Biochemistry. 2017 Dec 21;: Authors: Breindel LM, DeMott CM, Burz DS, Shekhtman A Abstract It is not well understood how ribosome antibiotics affect a wide range of biochemical pathways; changes in RNA-mediated protein quinary interactions and consequent activity inside the crowded cytosol may provide one possible mechanism. We developed real-time...	nmrlearner	Journal club	0	12-22-2017 07:55 PM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy. Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy. Angew Chem Int Ed Engl. 2015 Mar 5; Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S Abstract Altered metabolism is a critical part of...	nmrlearner	Journal club	0	03-11-2015 09:59 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off