2D-NMR could be a powerful complementary technique for assessing biosimilarity of protein drugs - News-Medical.net

		BioNMR > NMR community > Online News
2D-NMR could be a powerful complementary technique for assessing biosimilarity of protein drugs - News-Medical.net

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-10-2016, 09:28 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

2D-NMR could be a powerful complementary technique for assessing biosimilarity of protein drugs - News-Medical.net

2D-NMR could be a powerful complementary technique for assessing biosimilarity of protein drugs - News-Medical.net

News-Medical.net

2D-NMR could be a powerful complementary technique for assessing biosimilarity of protein drugs
News-Medical.net
A first-ever interlaboratory study of four versions of a therapeutic protein drugâ??all manufactured from living cellsâ??reports that an established analytical tool akin to magnetic resonance imaging reliably assessed the atomic structures of the ...

Read here

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Assessing the biosimilarity of protein drugs: New study shows method's precision - Phys.Org Assessing the biosimilarity of protein drugs: New study shows method's precision - Phys.Org http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcQRw4Xv7DAonuTByRXOGoGrmYZNiYJ7D1yAWHUqcRsVsXFRiUd3TmoRj-k0_B-rqRqiljnFtTE Phys.Org <img alt="" height="1" width="1"> Assessing the biosimilarity of protein drugs: New study shows method's precision Phys.Org Monoclonal antibodies are the largest class of approved protein therapeutics in the world, and the ability to extend 2D-NMR methods to this class of therapeutic would represent an important landmark in their analytical...	nmrlearner	Online News	0	02-06-2016 03:05 AM
Novel Technique Represents Major Advance in Protein Structure Research - Genetic Engineering & Biotechnology News Novel Technique Represents Major Advance in Protein Structure Research - Genetic Engineering & Biotechnology News <img alt="" height="1" width="1"> Novel Technique Represents Major Advance in Protein Structure Research Genetic Engineering & Biotechnology News Led by U. of I. chemistry professor Chad Rienstra, Ph.D., the team published its study ("Experimental protein structure verification by scoring with a single, unassigned NMR spectrum") in the journal Structure. "We've taken a process that would take ... and more » Read here	nmrlearner	Online News	0	10-16-2015 01:44 PM
Using ITC Technique for Characterization of Protein-Protein Interactions - News-Medical.net Using ITC Technique for Characterization of Protein-Protein Interactions - News-Medical.net http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcSPV3efNTb3XMRYliY8vnCW7qC7xNsSnkJLHKLQbyk7-ZGnhpI_1OC_nTXOAGrrAU5GSwVsiSRz News-Medical.net <img alt="" height="1" width="1"> Using ITC Technique for Characterization of Protein-Protein Interactions News-Medical.net With each interaction, the protein structural changes occur through specific electrostatic and van der Waals interactions, the breaking and formation of hydrogen bonds and the burial of surfaces exposed to solvents. It is...	nmrlearner	Online News	0	06-24-2015 01:08 PM
Automated Method for Purifying Protein:Ligand Complexes - News-Medical.net Automated Method for Purifying Protein:Ligand Complexes - News-Medical.net http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcQutdD6NnjJDnC9but3zjmMB4bBDLXFw5LX7xJfJWUzs2t8oAX-oS63Npooi2n9ra6w4V88Fp8j News-Medical.net <img alt="" height="1" width="1"> Automated Method for Purifying Protein:Ligand Complexes News-Medical.net Common techniques for further analyzing protein:ligand complexes to gain structural information include X-ray crystallography, NMR, and cryo-EM. However, a lack of reproducible and robust separation techniques means the purification of protein:ligand ...	nmrlearner	Online News	0	05-20-2015 10:27 AM
Protein Purification Using Avanti JXN Series Solution - News-Medical.net Protein Purification Using Avanti JXN Series Solution - News-Medical.net http://www.bionmr.com//t2.gstatic.com/images?q=tbn:ANd9GcSiDiy4ZkLlLCAizzcnWmf_fApD5eHnQ_9E5kH5pA5m-Qh88-iPDk8BZnntQEVKYL0HEbJ8OABC News-Medical.net <img alt="" height="1" width="1"> Protein Purification Using Avanti JXN Series Solution News-Medical.net A number of proteomics applications including, in vitrobiochemical assays, mass spectrometry, NMR and X-ray crystallography involve the use of purified proteins. Proteins may be isolated through their overexpression in organisms such as yeast, bacteria ... and...	nmrlearner	Online News	0	05-19-2015 09:10 AM
Newly identified protein markers have potential to contribute to better ... - News-Medical.net <img alt="" height="1" width="1" /> Newly identified protein markers have potential to contribute to better ... News-Medical.net Levels of GlycA and GlycB were determined from a blood test called nuclear magnetic resonance (NMR) spectroscopy, which was developed to determine the number of lipid particles contained in different cholesterol parameters. Testing for GlycA and GlycB ... and more » Newly identified protein markers have potential to contribute to better ... - News-Medical.net More...	nmrlearner	Online News	0	03-31-2014 11:13 PM
Scientists determine structure of Ltn1 protein - News-Medical.net Scientists determine structure of Ltn1 protein - News-Medical.net <img alt="" height="1" width="1" /> Scientists determine structure of Ltn1 protein News-Medical.net Ltn1 was deemed too large for its structure to be determined by current nuclear magnetic resonance (NMR) technology, and, as the scientists know now, too flexible to allow the highly regular crystalline packing needed by X-ray crystallographers. "It's ... Read here	nmrlearner	Online News	0	01-17-2013 02:09 PM
Structure of key protein for cellular signal transduction elucidated - News-Medical.n Structure of key protein for cellular signal transduction elucidated - News-Medical.net <img alt="" height="1" width="1" /> Structure of key protein for cellular signal transduction elucidated News-Medical.net Using NMR spectroscopy, Professor Michael Sattler and his team elucidated the spatial structure of the Qua1 region of Sam68, which is responsible for the ... and more » Read here	nmrlearner	Online News	0	09-10-2010 12:58 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off