BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > NMR Questions and Answers
Advanced Search
Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
 
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Old 06-22-2006, 09:25 AM
Junior Member
 
Join Date: Jul 2005
Posts: 1
Points: 12, Level: 1
Points: 12, Level: 1 Points: 12, Level: 1 Points: 12, Level: 1
Level up: 23%, 38 Points needed
Level up: 23% Level up: 23% Level up: 23%
Activity: 0%
Activity: 0% Activity: 0% Activity: 0%
NMR Credits: 0
NMR Points: 12
Downloads: 0
Uploads: 0
Send a message via MSN to nbkoneru Send a message via Yahoo to nbkoneru
Default Answered:

Dear sir,

Why we are using radio frequency excluding other electro magnetic radiation.

thankx
Reply With Quote


Did you find this post helpful? Yes | No
Best Answer - Posted by R2-D2
Quote:
Originally posted by nbkoneru@Jun 22 2006, 04:25 AM
Dear sir,

Why we are using radio frequency* excluding other electro magnetic radiation.

thankx
[snapback]169[/snapback]
I am just learning all this NMR theory too, so if anyone reads this and Im misguided please correct me.

The nuclei used in biological NMR all have spin I=1/2. This means that there are 2I+1=2 energy levels possible. These two energy levels are the lower energy state, where the magnetic moments of the nucei are parrallel to the magnetic field, and the high energy state, where the nuclei are antiparrallel to the magnetic field. The difference between these two energy levels is given by the equation E=(gyromagnetic ratio)(plank's constant/2*pi)(magnetic field). So for a 16.4 T magnet, the difference in energy states for proton would equal 4.672e-25 J. Using E=hv you find that the frequency needed to excicte nuclei from the lower to upper energy level is 7.05e8 /s, which falls in the range of radio frequency. If you do this with all the other spin 1/2 nuclei (15N,13C,31P) you find that these fall within that range as well. So to make a long story short, radio frequency is used because it provides sufficient energy to make the transition from the lower energy state to the higher energy state needed for excitation of the nuclei during an experiment.

  #2  
Old 07-02-2006, 06:50 PM
Junior Member
 
Join Date: Jun 2006
Posts: 3
Points: 16, Level: 1
Points: 16, Level: 1 Points: 16, Level: 1 Points: 16, Level: 1
Level up: 31%, 34 Points needed
Level up: 31% Level up: 31% Level up: 31%
Activity: 0%
Activity: 0% Activity: 0% Activity: 0%
NMR Credits: 0
NMR Points: 16
Downloads: 0
Uploads: 0
Provided Answers: 1
Default

Quote:
Originally posted by nbkoneru@Jun 22 2006, 04:25 AM
Dear sir,

Why we are using radio frequency* excluding other electro magnetic radiation.

thankx
[snapback]169[/snapback]
I am just learning all this NMR theory too, so if anyone reads this and Im misguided please correct me.

The nuclei used in biological NMR all have spin I=1/2. This means that there are 2I+1=2 energy levels possible. These two energy levels are the lower energy state, where the magnetic moments of the nucei are parrallel to the magnetic field, and the high energy state, where the nuclei are antiparrallel to the magnetic field. The difference between these two energy levels is given by the equation E=(gyromagnetic ratio)(plank's constant/2*pi)(magnetic field). So for a 16.4 T magnet, the difference in energy states for proton would equal 4.672e-25 J. Using E=hv you find that the frequency needed to excicte nuclei from the lower to upper energy level is 7.05e8 /s, which falls in the range of radio frequency. If you do this with all the other spin 1/2 nuclei (15N,13C,31P) you find that these fall within that range as well. So to make a long story short, radio frequency is used because it provides sufficient energy to make the transition from the lower energy state to the higher energy state needed for excitation of the nuclei during an experiment.
Reply With Quote


1 out of 1 members found this post helpful. Did you find this post helpful? Yes | No
Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMRpipe Yahoo group] Re: carrier frequency as determined by bruker
Re: carrier frequency as determined by bruker Hi Dean, It has to do with chemical shift referencing. On some Bruker machines the 13C and 15N base frequencies are not set to the recommended ones as More...
NMRpipe Yahoo group news News from other NMR forums 0 11-09-2011 06:44 AM
[NMRpipe Yahoo group] Re: carrier frequency as determined by bruker
Re: carrier frequency as determined by bruker Hi Dean, It has to do with chemical shift referencing. On some Bruker machines the 13C and 15N base frequencies are not set to the recommended ones as More...
NMRpipe Yahoo group news News from other NMR forums 0 11-08-2011 02:28 AM
Radio frequency assisted homonuclear recoupling - A Floquet description of homonuclear recoupling via surrounding heteronuclei in fully protonated to fully deuterated systems
Radio frequency assisted homonuclear recoupling - A Floquet description of homonuclear recoupling via surrounding heteronuclei in fully protonated to fully deuterated systems Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 18 January 2011</br> Michal, Leskes , Ümit, Akbey , Hartmut, Oschkinat , Barth-Jan, van Rossum , Shimon, Vega</br> We present a Floquet theory approach for the analysis of homonuclear recoupling assisted by radio frequency (RF) irradiation of surrounding heteronuclear spins. This description covers a...
nmrlearner Journal club 0 01-19-2011 03:04 PM
Decoupling, what modultaion frequency to use ?
Hi all, Perhaps a stupid question, but so far no one answered this one to me. I want to find out what decoupler modulation frequency (dmf in Varian syntax) is needed to properly decouple observed couplings. Can anyone tell me ? I am looking for a practical answer. I found a paper on decoupling, i.e. Shaka, A.J., Keeler, J. (1987): Progr. NMR Spectroscopy, 19, 47-129. Although this paper is formally correct, it is also very hard to read. So let's make a practical example: On an 800MHz machine, the carrier is on water (4.5 ppm), and there are imino protons resonating at 12-14 ppm....
Ramon989 NMR Questions and Answers 1 12-13-2010 06:56 AM
[NMR900 blog] Radio-Canada: interview with David Bryce
Radio-Canada: interview with David Bryce Les Chercheurs Dans le cadre de notre série sur les Chercheurs, nous recevons cette semaine David Bryce, professeur agrégé au Département de chimie et chercheur au Centre de recherche et d'innovation en catalyse de l'Université d'Ottawa (Radio-Canada).https://blogger.googleusercontent.com/tracker/8663203727601106205-4383726327099642978?l=nmr900.blogspot.com Read complete story on NMR900 blog
nmrlearner News from NMR blogs 0 08-22-2010 02:18 AM
NMR what is the resonance frequency of 1H nuclei if...?
you have just ran a H NMR spectrum of a compound on spectrometer with a 9.4T magnet, and the spectrum contains a peak at 3 ppm.also what is the chemical shift of these nuclei in HzThank you
Jonathan9303 NMR Questions and Answers 2 11-03-2005 03:55 PM



Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 02:30 PM.


Map