Unanswered: Too high automatic receiver gain value
I'm using "Bruker Avance 300" spectrometer ("Topspin"processing software). The problem is that when using automatic receiver gain aquisition option, it shows values about 3000 and more, not depending on sample concentration. What could cause this problem and how it can be fixed?
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[Question from NMRWiki Q&A forum] How to adjust the receiver gain in a deuterium oxide protein sample?
How to adjust the receiver gain in a deuterium oxide protein sample?
I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives a value of >10000 for the receiver gain. I understand this is happening because there is no water signal and hence the receiver gain is optimizing the S/N. However, should the experiment be run with that high value or should it be adjusted to a value of a size similar to the one used on water samples?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
11-28-2013 05:18 PM
[NMR paper] High-dimensionality (13)C direct-detected NMR experiments for the automatic assignment of intrinsically disordered proteins.
High-dimensionality (13)C direct-detected NMR experiments for the automatic assignment of intrinsically disordered proteins.
Related Articles High-dimensionality (13)C direct-detected NMR experiments for the automatic assignment of intrinsically disordered proteins.
J Biomol NMR. 2013 Nov 8;
Authors: Bermel W, Felli IC, Gonnelli L, Ko?mi?ski W, Piai A, Pierattelli R, Zawadzka-Kazimierczuk A
Abstract
We present three novel exclusively heteronuclear 5D (13)C direct-detected NMR experiments, namely (H(N-flip)N)CONCACON, (HCA)CONCACON and...
nmrlearner
Journal club
0
11-11-2013 01:30 AM
[Question from NMRWiki Q&A forum] Sensitivity gain by utilizing steady state polarization
Sensitivity gain by utilizing steady state polarization
What do we exactly mean by "utilizing steady state polarization" to gain sensitivity in an experiment?...I know that steady state should be achieved before the start of acquisition which means, in a way, it is already being used for 1H,13C/15N(isn't it?)...I am lost in understanding this concept!...also, how/why does it differ in standard pulse sequences and spin state selective coherence transfer experiments?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
08-05-2013 09:06 AM
UW Students Gain Research Experience as Undergraduates - University of Wyoming News
http://www.bionmr.com//nt3.ggpht.com/news/tbn/TwnxFHMkKxrmoM/6.jpg
University of Wyoming News
<img alt="" height="1" width="1" />
UW Students Gain Research Experience as Undergraduates
University of Wyoming News
They use Nuclear Magnetic Resonance spectroscopy and other biophysical methods to study protein structure and function. Moulton is a member of the American Chemical Society, and has been recognized as a "Superior Student" in the UW Department of ...
UW Students Gain Research Experience as Undergraduates - University of Wyoming News
nmrlearner
Online News
0
04-20-2013 02:07 AM
[U. of Ottawa NMR Facility Blog] Receiver Gain and Signal-to-Noise Ratio
Receiver Gain and Signal-to-Noise Ratio
The signal-to-noise ratio in an NMR spectrum can be affected drastically the choice of the receiver gain setting, so care should be taken to set the receiver gain correctly for optimum results. At very low receiver gain settings, both the signal and the noise use only a fraction of the available digitization levels of the analog-to-digital concertor (ADC). As a result, the intensity of each point in the FID is represented with only a few possible values and the FID is "choppy". This is analogous to a black and white photograph being represented...
nmrlearner
News from NMR blogs
0
02-15-2013 04:27 AM
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
Abstract While NMR studies of proteins typically aim at structure, dynamics or interactions, resonance assignments represent in almost all cases the initial step of the analysis. With increasing complexity of the NMR spectra, for example due to decreasing extent of ordered structure, this task often becomes both difficult and time-consuming, and the recording of high-dimensional data with high-resolution may be essential. Random sampling of the evolution time space,...
nmrlearner
Journal club
0
07-20-2012 11:13 PM
[Question from NMRWiki Q&A forum] Switiching off the receiver during acquisition
Switiching off the receiver during acquisition
Hello nmr wikier. I use Bruker Avance I AQS 400. TopSpin Version 1.3
For a test I want to switch off the receiver during the acquisition. In pursuance of the pulse programming manual I can do that with the statement setrtp.
So I wrote following pulse programm (excerpt):
1 ze
2 30m
p1 ph1
3 DE1 DE2 DERX DEADC DEPA DE3
4 0.1u DWELL_GEN:f1
nmrlearner
News from other NMR forums
0
09-13-2011 09:15 PM
Towards automatic metabolomic profiling of high-resolution one-dimensional proton NMR spectra
Towards automatic metabolomic profiling of high-resolution one-dimensional proton NMR spectra
Abstract Nuclear magnetic resonance (NMR) and Mass Spectroscopy (MS) are the two most common spectroscopic analytical techniques employed in metabolomics. The large spectral datasets generated by NMR and MS are often analyzed using data reduction techniques like Principal Component Analysis (PCA). Although rapid, these methods are susceptible to solvent and matrix effects, high rates of false positives, lack of reproducibility and limited data transferability from one platform to the next....
Linear Mode
