Asking about Deuterium NMR

[santoshbharti]

I wanted to do Deuterium NMR in human urine sample but no one has done previously in our lab.
In our Lab. we have 400 MHz Bruker (Avance) spectrometer with BroadBand Inverse probe (1H Observe, Z-gradient).
How we have to proceed without deuterium lock which is neccessory for exp. ?
Is above described instruments specification sufficient to perform the deutrium NMR experiments ?

Please any on can help


Thanks very much

[sivanmr]

Do you have your human urine sample labeled with deuterium !!! Just kidding.... All you have to do is switch off the lock using command "locnuc=off" and use the sequence ZG2H (read the sequence carefully). Well, this is a general instruction to run 2H spectrum in a Bruker spectrometer. If you fry the probe without taking proper care, i am not responsible for it !!!

[santoshbharti]

Thanks Man,
Is it require proton decoupling ?
if yes.
then tell me that zgdc or zgpg pulse sequence can be used or not ?

[ptrigomou]

An alternative method to switching off the D-Lock is the use of capilar tubes, I use a NewEra's capilar tube for 500 µL of sample and 60 µL of D2O in the capilar