Sequential Assignment of Spin systems

hi,
I am new to the protein NMR Field.I want to assign spin numbers to 120 amonoacid residues of a protein.I am cluless how to assing these number to each.Could anyone help me? Here is the primary sequence f a protein domain-

ISLLAQRQQF WIQLEFHSRI TRGERHGVID HVGLGVQSQQ RSNGFLTSLC ILRPHYASLS LALEKAQLHS LLCEETDGEG TLEYGFMGQV KSRFTDLSRP NLFCRHLGQL LPLLRVCRDV

I am expecing your help.Thank you.

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03-13-2009, 03:14 PM

Steve

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Hi, you don't provide much information in your post - sequentially assigning a 120 amino acid protein can be quite a job.

Could you reply with what experiments you've run, and then we could try again?

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Sequential Assignment of a proteing sequence hi, I am new to the protein NMR Field.I want to assign spin numbers to 120 amonoacid residues of a protein.I am cluless how to assign these number to each.Could anyone help me? Here is the primary sequence of a protein domain- ISLLAQRQQF WIQLEFHSRI TRGERHGVID HVGLGVQSQQ RSNGFLTSLC ILRPHYASLS LALEKAQLHS LLCEETDGEG TLEYGFMGQV KSRFTDLSRP NLFCRHLGQL LPLLRVCRDV I am expecing your help.Could any one do it on some software program and send to me in my email pkp08@yahoo.com.Thank you.	ppaudyal	A test forum	0	02-16-2009 03:35 AM

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