Nmrd

[Vladimir]

Hello!

Can someone explain to me how NMRD (nuclear magnetic relaxation dispersion) works? I found many papers where this technique was used but I could not find any explanations / pulse sequences for it. Essentially all I know is that you get a graph (vCPMG versus the transverse relaxation rate), from which you can extract information about time dynamic behaviour. So anything you can tell me is very welcome.
Also I don't know what the vCPMG frequency is (CPMG = Carr-Purcell-Meiboom-Glll).

best regards
Vladimir

[mrevingt]

A good early paper that goes into some detail about the parameters and theory is:

Measurement of Slow (s-ms) Time Scale Dynamics in Protein Side Chains by 15N Relaxation Dispersion NMR Spectroscopy: Application to Asn and Gln Residues in a Cavity Mutant of T4 Lysozyme
Frans A. A. Mulder, Nikolai R. Skrynnikov, Bin Hon, Frederick W. Dahlquist, and Lewis E. Kay*
J. Am. Chem. Soc., 123 (5), 967 -975, 2001. 10.1021/ja003447g S0002-7863(00)03447-8
Web Release Date: January 16, 2001

although it is aimed at only at sidechain amide groups.

A more comprehensive review can be found at:

A.G. Palmer, C.D. Kroenke and J.P. Loria, NMR methods for quantifying microsecond-to-millisecond motions in biological macromolecules, Methods Enzymol. 339 (2001), pp. 204–238.

Hope this helps