Can you please explain the meaning of splitting in a C-13 NMR spectra mean?

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Can you please explain the meaning of splitting in a C-13 NMR spectra mean?

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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11-25-2003, 07:58 AM

alex k

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Answered: what does splitting in a C-13 NMR spectra mean?

I know what splititng in a Proton NMR spectrum indicates, but if you see it in C13 NMR then what does that indicate?

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Best Answer - Posted by Mark R

Normally 13 C NMR spectra are run proton decoupled to simplify the spectra and enhance the intensity of the peaks. However, the decoupler is set for protons not deuterons so you will see splitting for the solvent you are using like CDCl3 or d6DMSO etc
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11-25-2003, 07:58 AM

Mark R

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what does splitting in a C-13 NMR spectra mean?

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