Unanswered: arginine side chain assignment pulse wanted
Hi, I want use 15N labeling for arginine side chain assignment. it seems the 2D HE(NE)HGHH is the right pulse to use ( J bio. NMR. 10(1997):193 ). You will be very appreciated for any information about getting this pulse. Thanks.
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[Question from NMRWiki Q&A forum] Side chain assignment of C-terminal residue
Side chain assignment of C-terminal residue
Dear Friends,
I am not able to figure out how to determine the side chain assignment of Last C-terminal SERINE residue of my protein. I can determine CA, CB, CO, N,H values from HNCA, CBCANH, HNCACO. Can someone tell which experiment will give me the information of HA, HB2 and HB3
Regards
Arun
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