The following Bruker pulse sequences can be found on BMRB website.
Shift correlation:
Homonuclear
Three-dimensional
TOCSY-HSQC 3D experiment
NOESY-HSQC 3D experiment
Heteronuclear
Two-dimensional
Bruker DMX 15N-1H 2D HSQC with Sensitivity-enhanced
Bruker AMX 15N-1H 2D HSQC with Sensitivity-enhanced
HMBC with gradient selection of echo and antiecho in t1 for suppression of t1-noise.
hsqc employing gradients for coherence selection. Phase sensitive by sampling +/- coherence in alternate scans.
selective hmbc with gradients; used when the selective 180 is greater than approx 1.5 ms
selective hmbc with gradients; used when the selective 180 is less than approx 1.5 ms
hsqc with selective 180(1H) pulses for high S/N
Three-dimensional
3D HNCaCb - Gradient enhanced
3D HNCaCb - Gradient enhanced with minimal H2O saturation
3D HNCA with Gradient sensitivity enhancement with minimal H2O saturation
3D HNCO with Gradient sensitivity enhancement with minimal H2O saturation
3D HN(CO)CA with gradient sensitivity enhancement and minimal H2O saturation
3D HCAN, gradient enhanced, deuterium decoupled
- gradient program for previous pulse sequence
- include file for previous pulse sequence
3D HCA(CO)N, gradient enhanced, deuterium decoupled
- gradient program for previous pulse sequence
- include file for previous pulse sequence
Exchange spectroscopy (cross-relaxation and chemical exchange):
Homonuclear
One-dimensional
13C edited (HMQC) 1D NOE difference JR for water suppression (9/27/95)
Two-dimensional
symmetrized CW-ROESY, program for DMX hardware, tested on DMX 500i and DMX750
Three-dimensional
Block-decoupled diagonal elements with spin diffusion eliminated 3D NOESY
Block-decoupled off-diagonal elements with spin diffusion eliminated 3D NOESY
Four-dimensional
4D (13C,13C)-HMQC-NOESY-HSQC
Heteronuclear
Two-dimensional
2D 1H/15N heteronuclear NOE with water along +Z
Three-dimensional
Bruker AMX 15N-1H 3D NOESY-HSQC with Sensitivity-enhanced
Relaxation
T1 Relaxation
DMX using States-TPPI
T1rho Relaxation
Off-resonance T1rho experiment
DMX using States-TPPI
J-coupling
Homonuclear
Two-dimensional
2D difference 13C CT-HSQC for determination of J3(C'Cb) in Val, Ile, and Thr residues for the determination of X1 angle
Heteronuclear
Two-dimensional
Bruker DMX non-gradient 2D CT-HSQC; reference for 3D HNHB
Bruker DMX gradient 2D CT-HSQC; reference for 3D gradient HNHB
Auxiliary program needed by gradient 2D CT-HSQC
Measurement of 3J(HnHa) from a set of 2D J-modulated HMQC spectra.
2D difference 13C CT-HSQC for determination of J3(NC) in Val, Ile, and Thr residues for the determination of X1 angle
Three-dimensional
Bruker DMX non-gradient 3D HNHB
Bruker DMX gradient 3D HNHB
Auxiliary program needed by gradient 3D HNHB
Measurement of 3J(HaC') from E.COSY splitting.
Measurement of 3J(HnCb) from E.COSY splitting.
Measurement of 3J(HnHa) from E.COSY splitting.
Measurement of C' to H J couplings from quantitative-type measurement.
Dipolar coupling
Heteronuclear
Two-dimensional
2D IPAP [15N, 1H] HSQC
2D IPAP HNCO
Three-dimensional
3D J-modulated HSQC
3D HNCA gradient enhanced
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[NMR paper] Sequential HNCACB and CBCANH protein NMR pulse sequences.
Sequential HNCACB and CBCANH protein NMR pulse sequences.
Related Articles Sequential HNCACB and CBCANH protein NMR pulse sequences.
J Magn Reson. 2001 Aug;151(2):328-31
Authors: Meissner A, Sørensen OW
The pulse sequences HNCACB and CBCANH correlating side chain C(beta) resonances with amide resonances in the protein backbone do not distinguish between inter- and intraresidue correlations. The new pulse sequences sequential HNCACB and sequential CBCANH make this distinction by suppressing coherence transfer between 13C(alpha) and 15N via the...
nmrlearner
Journal club
0
11-19-2010 08:44 PM
[Question from NMRWiki Q&A forum] Varian Biopack pulse sequences for intermolecular NOEs
Varian Biopack pulse sequences for intermolecular NOEs
Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the moment from looking at the Biopack library, I have found 4 pulse sequences which will do this and each provide different information (gnoesyNhsqc_CN, gnoesyNhsqc_NN, gnoesyChsqc_CC and gnoesyChsqc_NC). Would people recommend using a combination of all four of these experiments to determine the intermolecular NOEs for use in structure calculations?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] Bruker Avance pulse sequences - where to find if not in TopSpin?
Bruker Avance pulse sequences - where to find if not in TopSpin?
I used to use Varian Inova systems with BioPack all the time, and now I'm using a Bruker Avance-III system geared for Chemistry, not biology. It's driving me crazy that I can't find the pulse sequences I'm used to using! Right now, I'd like to use a NOESY with WET water suppression, and I can't find one in the default set that came with the software.
Where do I go for relatively simple pulse sequences for Avance systems? I see Bruker pulse sequences for advanced experiments on Ad Bax's site, at NMRFAM, and NMRWiki, but...
nmrlearner
News from other NMR forums
0
08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] How do you port pulse sequences to different field strength?
How do you port pulse sequences to different field strength?
Hi, when you set up a pulse sequence at a different magnet field strength - starting with an existing set of parameters - what do you look at?
Does length of shaped pulses always need to be changed?
What pulse lenghts need to be adjusted?
When it is necessary to re-build RF waveforms - for what kinds of pulses?
nmrlearner
News from other NMR forums
0
08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] Are hardcoded values in bruker pulse sequences overridable?
Are hardcoded values in bruker pulse sequences overridable?
For example when there is a statement like this in the pulse program file
"d12=20u" can I change value of d12 from the command line?
Thanks.
nmrlearner
News from other NMR forums
0
08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] 2D pulse sequences for NMR of protein/peptide complexes?
2D pulse sequences for NMR of protein/peptide complexes?
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone have any ideas where I would get some tried and tested pulse sequence input files?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: Are hardcoded values in #bruker #nmr pulse sequences overridable? http://qa.
nmrwiki: Are hardcoded values in #bruker #nmr pulse sequences overridable? http://qa.nmrwiki.org/question/147/
nmrwiki: Are hardcoded values in #bruker #nmr pulse sequences overridable? http://qa.nmrwiki.org/question/147/
Source: NMRWiki tweets
nmrlearner
Twitter NMR
0
08-22-2010 01:49 AM
NMR pulse sequences of Danish Instrument Center
The following NMR pulse sequences for biological macromolecules are available on the website of the Danish Instrument Center as of 3/15/05: Coupling Constants in Proteins
- 2D S3CT for measurement of one bond coupling constants (J & RDC) - Varian, Bruker
TROSY experiments
- clean TROSY, 15N-1H correlation, gradient version, water flip back, optional t2 decoupling - Varian, Bruker
- constant-time S3CT TROSY, 13 C-1H correlation, gradient version - Varian, Bruker
- (H)CCH TROSY, 3D TROSY-type HCCH correlation with diagonal peak suppression - Varian
- TROSY-Type NOESY, 2D NOESY with...
Linear Mode
