where ‘brd’ refers to broadening, and all other parameters have ...
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18/06/2013 7:23:35 AM GMT
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[U. of Ottawa NMR Facility Blog] Exponential Line Broadening - Video Tutorial
Exponential Line Broadening - Video Tutorial
Exponential line broadening is an important NMR data processing tool. It involves multiplying the time domain signal by a decaying exponential function prior to Fourier transforming the data into the frequency domain. It is used to improve the signal-to-noise ratio and is more fully described in a previous post. The following short tutorial video demonstrates its use.
http://4.bp.blogspot.com/-CozKHXyqcTA/UUdrJzrbyqI/AAAAAAAABG8/0FLtL9-4af0/s400/lb_vid.jpg
nmrlearner
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03-19-2013 12:58 AM
[Question from NMRWiki Q&A forum] File with description of parameters
File with description of parameters
Hi, comrades! We use the linux version of TopSpin 2.1 as a software for AVANCE II Bruker 400 MHz.As I found parameters of pulse sequences are located in /opt/topspin/exp/stan/nmr/par - directory.
I've started to study the content of "acqu" - file in directory /opt/topspin/exp/stan/nmr/par/PROTON and couldn't find any definitions of some parameters in Bruker-manuals. For instance, DBL, DATE, DBP, DBPNAME, DECBNUC, DECNUC, DECSTAT, DL, DP, DPO.
Where can I find the meaning and explanation of that parameters?
nmrlearner
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10-02-2012 06:32 PM
[Question from NMRWiki Q&A forum] residue information in case of line broadening (intensity drop ) of HSQC titration
residue information in case of line broadening (intensity drop ) of HSQC titration
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is complex size 33 kd .
is their any method to extract...
nmrlearner
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05-11-2012 10:36 AM
[Question from NMRWiki Q&A forum] How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is...
nmrlearner
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05-09-2012 07:38 PM
[Question from NMRWiki Q&A forum] Line broadening in nmr
Line broadening in nmr
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small molecules and I observed severe broadening at a protein to ligand ratio 1:20. I am pretty sure that its not aggregation since i have the free ligand spectrum without the protein which didnt showed any broadening effect.
Hope somebody will guide me..
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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10-04-2011 08:47 PM
[Question from NMRWiki Q&A forum] adjusting topshim parameters
adjusting topshim parameters
On our Avance III 500 we use Topshim quite successfully for any probe with a gradient coil. The only time we have a problem is with CDCl3 solvent on our 1.7 mm TXI probe, where Topshim fails with a complaint about the signal to noise ratio being too low. This is not surprising for a microprobe, as CDCl3 has the lowest signal of any common deuterated solvent. We have confirmed that the 2H pulse length is correct in the prosol file.
An obvious solution would be to increase the nunber of scans for this probe/solvent combination. There seem to be probe and...
nmrlearner
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07-25-2011 11:01 AM
[Question from NMRWiki Q&A forum] same parameters but difference in phase why?
same parameters but difference in phase why?
hi, though this question seems to be easy for you but i am new for this software and to this field. Recently, i took silicon spectra in 500mhz(bruker) seems to be good (i.e.one silicon peak and one nmr tubes broad peak) but when i used same parameters for another sample where i got single peak as i expected but nmr tube peak is inverse this time. I just copied previous parameters only why i got such a inverse signal.If anybody know please help me.
thank you
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nmrlearner
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03-31-2011 09:21 PM
[NMR paper] NMR exchange broadening arising from specific low affinity protein self-association:
NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.
Related Articles NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.
J Biomol NMR. 1999 Aug;14(4):307-20
Authors: Pfuhl M, Chen HA, Kristensen SM, Driscoll PC
Nuclear spin relaxation monitored by heteronuclear NMR provides a useful method to probe the overall and internal molecular motion for...
where ‘brd’ refers to broadening, and all other parameters have ...
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