Domain cooperativity in multidomain proteins: what can we learn from molecular alignment in anisotropic media?
Domain cooperativity in multidomain proteins: what can we learn from molecular alignment in anisotropic media?
Abstract Many proteins have modular design with multiple globular domains connected via flexible linkers. As a simple model of such system, we study a tandem construct consisting of two identical SH3 domains and a variable-length Gly/Ser linker. When the linker is short, this construct represents a dumbbell-shaped molecule with limited amount of domainā??domain mobility. Due to its elongated shape, this molecule efficiently aligns in steric alignment media. As the length of...