NMR images ... way to measure this coupling constant in unlabeled proteins is fitting

		BioNMR > NMR community > NMR pictures
[NMR images] ... way to measure this coupling constant in unlabeled proteins is fitting

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-20-2014, 12:44 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

... way to measure this coupling constant in unlabeled proteins is fitting

20/03/2014 12:45:23 PM GMT
... way to measure this coupling constant in unlabeled proteins is fitting
More...

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
On the calculation of 3JÎ±Î²-coupling constants for side chains in proteins On the calculation of 3JÎ±Î²-coupling constants for side chains in proteins Abstract Structural knowledge about proteins is mainly derived from values of observables, measurable in NMR spectroscopic or X-ray diffraction experiments, i.e. absorbed or scattered intensities, through theoretically derived relationships between structural quantities such as atom positions or torsional angles on the one hand and observable quantities such as squared structure factor amplitudes, NOE intensities or 3 J-coupling constants on the other. The standardly used relation connecting 3 J-couplings...	nmrlearner	Journal club	0	06-25-2012 04:41 AM
More accurate 1JCH coupling measurement in the presence of 3JHH strong coupling in natural abundance More accurate 1JCH coupling measurement in the presence of 3JHH strong coupling in natural abundance Publication year: 2012 Source:Journal of Magnetic Resonance, Volume 215</br> Bingwu Yu, Hugo van Ingen, Subramanian Vivekanandan, Christoph Rademacher, Scott E. Norris, Darón I. Freedberg</br> J couplings are essential for measuring RDCs (residual dipolar couplings), now routinely used to deduce molecular structure and dynamics of glycans and proteins. Accurate measurement of 1 J CH is critical for RDCs to reflect the true structure and dynamics in the molecule of...	nmrlearner	Journal club	0	03-09-2012 09:16 AM
The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. Top Curr Chem. 2011 Sep 28; Authors: Chen K, Tjandra N Abstract The development of residual dipolar coupling (RDC) in protein NMR spectroscopy, over a decade ago, has become a useful and almost routine tool for accurate protein solution structure determination. RDCs provide orientation information of magnetic dipole-dipole interaction vectors within a common reference frame. Its measurement requires a...	nmrlearner	Journal club	0	09-30-2011 06:00 AM
The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. Top Curr Chem. 2011 Sep 28; Authors: Chen K, Tjandra N Abstract The development of residual dipolar coupling (RDC) in protein NMR spectroscopy, over a decade ago, has become a useful and almost routine tool for accurate protein solution structure determination. RDCs provide orientation information of magnetic dipole-dipole interaction vectors within a common reference frame. Its measurement requires a...	nmrlearner	Journal club	0	09-30-2011 05:59 AM
[Question from NMRWiki Q&A forum] How can I calculate a carbon-proton coupling constant for a molecule? How can I calculate a carbon-proton coupling constant for a molecule? I'm trying to explain a missing HMBC peak, and having a coupling constant less than 10 Hz would do that nicely. It's a formamidine derivative with a 3 bond correlation N=CHNC The C is a quaternary carbon in a benzene ring. Any help would be appreciated. Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	02-04-2011 07:12 PM
[NMR paper] Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically l Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Related Articles Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol. 2005 Jun;23(6):736-40 Authors: Züger S, Iwai H Segmental isotopic labeling of proteins using protein ligation is a recently established in vitro method for incorporating isotopes into one domain or region of a protein to reduce the complexity of NMR spectra, thereby facilitating...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] An efficient NMR experiment for analyzing sugar-puckering in unlabeled DNA: applicati An efficient NMR experiment for analyzing sugar-puckering in unlabeled DNA: application to the 26-kDa dead ringer-DNA complex. Related Articles An efficient NMR experiment for analyzing sugar-puckering in unlabeled DNA: application to the 26-kDa dead ringer-DNA complex. J Magn Reson. 2001 Dec;153(2):262-6 Authors: Iwahara J, Wojciak JM, Clubb RT We present a new NMR experiment for estimating the type and degree of sugar-puckering in high-molecular-weight unlabeled DNA molecules. The experiment consists of a NOESY sequence preceded by a...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] A structural mode-coupling approach to 15N NMR relaxation in proteins. A structural mode-coupling approach to 15N NMR relaxation in proteins. Related Articles A structural mode-coupling approach to 15N NMR relaxation in proteins. J Am Chem Soc. 2001 Apr 4;123(13):3055-63 Authors: Tugarinov V, Liang Z, Shapiro YE, Freed JH, Meirovitch E The two-body Slowly Relaxing Local Structure (SRLS) model was applied to (15)N NMR spin relaxation in proteins and compared with the commonly used original and extended model-free (MF) approaches. In MF, the dynamic modes are assumed to be decoupled, local ordering at the N-H sites...	nmrlearner	Journal club	0	11-19-2010 08:32 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off