[Question from NMRWiki Q&A forum] no idea what going on?
no idea what going on?
I am trying to set up a 3d 1h detected exp in solid. working on 700 MHz spectrometer,with a 3.2 mm probe. normal zg on 13c, is good ( so sample is fine). the problem occurs that i have two cp( cross polarisation) transfers steps in my 3d exp, I am not able to get 1D 1H signal out of 3d exp.the exp with the cp works well on 600 MHz spectrometer though. is it a probe problem or a magnetic field inhomogeneity? any clue will be appreciated.
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
02-20-2013 06:24 PM
[NMR paper] A solid-state NMR index of helical membrane protein structure and topology.
A solid-state NMR index of helical membrane protein structure and topology.
Related Articles A solid-state NMR index of helical membrane protein structure and topology.
J Magn Reson. 2000 May;144(1):150-5
Authors: Marassi FM, Opella SJ
The secondary structure and topology of membrane proteins can be described by inspection of two-dimensional (1)H-(15)N dipolar coupling/(15)N chemical shift polarization inversion spin exchange at the magic angle spectra obtained from uniformly (15)N-labeled samples in oriented bilayers. The characteristic...
nmrlearner
Journal club
0
11-18-2010 09:15 PM
[NMR paper] 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Esch
1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein.
Eur J Biochem. 1997 Jun 1;246(2):301-10
Authors: Beglova N, Fischer D, Hengge-Aronis R, Gehring K
GlgS is a 7892-Da protein which is involved in glycogen...
nmrlearner
Journal club
0
08-22-2010 03:31 PM
[NMR paper] 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Esch
1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein.
Eur J Biochem. 1997 Jun 1;246(2):301-10
Authors: Beglova N, Fischer D, Hengge-Aronis R, Gehring K
GlgS is a 7892-Da protein which is involved in glycogen...
nmrlearner
Journal club
0
08-22-2010 03:03 PM
[NMR paper] 1H resonance assignments, secondary structure and general topology of single-chain mo
1H resonance assignments, secondary structure and general topology of single-chain monellin in solution as determined by 1H 2D-NMR.
Related Articles 1H resonance assignments, secondary structure and general topology of single-chain monellin in solution as determined by 1H 2D-NMR.
J Biomol NMR. 1992 Nov;2(6):557-72
Authors: Tomic MT, Somoza JR, Wemmer DE, Park YW, Cho JM, Kim SH
We determined the resonance assignments, secondary structure and general topology of the 11-kDa sweet protein single-chain monellin (SCM), using two-dimensional proton...
nmrlearner
Journal club
0
08-21-2010 11:45 PM
[NMR paper] Secondary structure and topology of interleukin-1 receptor antagonist protein determi
Secondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopy.
Related Articles Secondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopy.
Biochemistry. 1992 Jun 16;31(23):5237-45
Authors: Stockman BJ, Scahill TA, Roy M, Ulrich EL, Strakalaitis NA, Brunner DP, Yem AW, Deibel MR
Interleukin-1 (IL-1) proteins, such as IL-1 beta, play a key role in immune and inflammatory responses....
nmrlearner
Journal club
0
08-21-2010 11:41 PM
[NMR paper] Determination of the secondary structure and molecular topology of interleukin-1 beta
Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy.
Related Articles Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy.
Biochemistry. 1990 May 15;29(19):4668-82
Authors: Driscoll PC, Gronenborn AM, Wingfield PT, Clore GM
A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been...