[Question from NMRWiki Q&A forum] what is best pulse sequence for c13 edit noesy on vnmrj ?
what is best pulse sequence for c13 edit noesy on vnmrj ?
Hi
What is best pulse sequence in bio pack on vnmrj for C-13 edit NOESY ? I am working with 12 kd size protein which can exist as homo dimers ( i.e total size of homo-dimer is 24 kd ). Most frequent problems we are encountering as follows !line broadening , baseline , phase correction problems ( huge signal at 2.8 ppm )in first increments and f2f3 planes huge strips errors !
Thanking you in advance !
nmrlearner
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01-10-2012 03:38 PM
[Question from NMRWiki Q&A forum] Problem while genrating the constraints form N-15 edit AND C-13 EDIT NOESY files
Problem while genrating the constraints form N-15 edit AND C-13 EDIT NOESY files
I am encountering the problem while generating the constraints form N-15 edit AND C-13 EDIT NOESY files that very few constraints 200 out of 2400 peaks .Calibration is OK .chemical shift file is good agreement with secondary structure of know crystal structure .Completeness of assignment:Completeness of all atom assignment is 80.74 %.Completeness of proton atom assignment is 82.50 %.Completeness of heavy atom assignment is 74.56 %.Completeness of all atom assignment is 74.58 %.Completeness of proton atom...
nmrlearner
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12-24-2011 12:27 AM
[Question from NMRWiki Q&A forum] c13 edit noesy phasing problem
c13 edit noesy phasing problem
Dear NMR Wiki'ers
I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of 120ms , I tried my best to correct the phasing, still it seems to be negative contours ,Peak broadening and noise is more after processing .How to overcome this problem ?
Fig-1 1D projections fig-2 f1f3 projection in process in t1fig-3 f1f3 projection in process in t1 after transformation (1026-512-256)
<img width=50% src="http://qa.nmrwiki.org/upfiles/13204074491921939.png" /img>
nmrlearner
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11-04-2011 04:53 PM
[Question from NMRWiki Q&A forum] Auto assignment of N15 C13 EDIT noesy experments
Auto assignment of N15 C13 EDIT noesy experments
Dear NMR wikiers
could you please suggest any auto assignment web servers online to assign N-15 & C13- EDIT NOESY experiments data?
Thanking you