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9/02/2011 8:21:55 AM GMT
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Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data
Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data
Abstract One bottleneck in NMR structure determination lies in the laborious and time-consuming process of side-chain resonance and NOE assignments. Compared to the well-studied backbone resonance assignment problem, automated side-chain resonance and NOE assignments are relatively less explored. Most NOE assignment algorithms require nearly complete side-chain resonance assignments from a series of through-bond experiments such as HCCH-TOCSY or HCCCONH. Unfortunately, these TOCSY...
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06-27-2011 04:30 AM
[Question from NMRWiki Q&A forum] assignment of 12 kd protein
assignment of 12 kd protein
Dear memers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone using HNCA, HNCOCA CBCANH and CBCACONH , still some peaks are not quality of cbcanh and cbcaconh . how to assign rest of them using other experments ( HCCONH, HBHACONH ,CCCONH ) ?iam using sparky , vnmrj 700 mhz
thanking you anticipation
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05-10-2011 07:17 PM
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11-25-2010 08:21 PM
[NMR paper] NvAssign: protein NMR spectral assignment with NMRView.
NvAssign: protein NMR spectral assignment with NMRView.
Related Articles NvAssign: protein NMR spectral assignment with NMRView.
Bioinformatics. 2004 May 1;20(7):1201-3
Authors: Kirby NI, DeRose EF, London RE, Mueller GA
MOTIVATION: Nuclear magnetic resonance (NMR) protein studies rely on the accurate assignment of resonances. The general procedure is to (1) pick peaks, (2) cluster data from various experiments or spectra, (3) assign peaks to the sequence and (4) verify the assignments with the spectra. Many algorithms already exist for...
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11-24-2010 09:25 PM
[Nature network NMR forum] Practical guide to protein NMR assignment (1 reply)
Practical guide to protein NMR assignment (1 reply)
There is a new website guiding new spectroscopists through the basics of assigning their favorite protein – click here
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08-21-2010 03:29 PM
NvAssign: protein NMR spectral assignment with NMRView
NvAssign: protein NMR spectral assignment with NMRView.
http://dir.niehs.nih.gov/dirnmr/nvassign/manual/images/examinePeaks.jpg
Designed by Kirby NI, DeRose EF, London RE, Mueller GA.
Laboratory of Structural Biology, MR-01, National Institute of Environmental Health Sciences, National Institutes of Health, Box 12233, Research Triangle Park, NC 27709, USA.
Published in Bioinformatics. 2004 May 1;20(7):1201-3. Epub 2004 Feb 10.