[NMR paper] Tracing Metabolite Footsteps of Escherichia coli Along the Time Course of Recombinant Protein Expression by Two-Dimensional NMR Spectroscopy.
Tracing Metabolite Footsteps of Escherichia coli Along the Time Course of Recombinant Protein Expression by Two-Dimensional NMR Spectroscopy.
Related Articles Tracing Metabolite Footsteps of Escherichia coli Along the Time Course of Recombinant Protein Expression by Two-Dimensional NMR Spectroscopy.
Bull Korean Chem Soc. 2012 Dec 20;33(12):4041-4046
Authors: Chae YK, Kim SH, Ellinger JJ, Markley JL
Abstract
The recombinant expression of proteins has been the method of choice to meet the demands from proteomics and structural genomics...
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06-26-2013 09:39 AM
Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor.
Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor.
Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor.
Protein Expr Purif. 2011 May 13;
Authors: Bellot G, Pascal R, Mendre C, Urbach S, Mouillac B, Déméné H
The vasopressin type 2 (V2R) receptor belongs to the class of G-protein coupled receptors. It is mainly expressed in the membrane of kidney tubules,...
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05-19-2011 04:20 AM
[NMR paper] Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloi
Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloid protein for solid-state NMR.
Related Articles Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloid protein for solid-state NMR.
Protein Expr Purif. 2005 Jul;42(1):200-10
Authors: Sharpe S, Yau WM, Tycko R
Fibrillar protein aggregates contribute to the pathology of a number of disease states. To facilitate structural studies of these amyloid fibrils by solid-state NMR, efficient methods for the production of milligram...
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11-24-2010 11:14 PM
[NMR paper] Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris
Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: detection of partial oxidation of methionine by NMR.
Related Articles Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: detection of partial oxidation of methionine by NMR.
Protein Expr Purif. 2004 Oct;37(2):336-43
Authors: Barral P, Tejera ML, Treviño MA, Batanero E, Villalba M, Bruix M, Rodríguez R
Olive pollen is one of the main causes of allergy in Mediterranean countries. Ole e 6, an olive pollen...
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11-24-2010 10:01 PM
[NMR paper] Dictyostelium discoideum as expression host: isotopic labeling of a recombinant glyco
Dictyostelium discoideum as expression host: isotopic labeling of a recombinant glycoprotein for NMR studies.
Related Articles Dictyostelium discoideum as expression host: isotopic labeling of a recombinant glycoprotein for NMR studies.
Protein Expr Purif. 2000 Aug;19(3):335-42
Authors: Cubeddu L, Moss CX, Swarbrick JD, Gooley AA, Williams KL, Curmi PM, Slade MB, Mabbutt BC
The advantages of the organism Dictyostelium discoideum as an expression host for recombinant glycoproteins have been exploited for the production of an isotopically...
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11-19-2010 08:29 PM
[NMR paper] Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermo
Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermodule: spectroscopic/functional individuality of plasminogen kringle domains.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Recombinant gene expression and 1H NMR characteristics of the kringle (2 + 3) supermodule: spectroscopic/functional individuality of plasminogen kringle domains.
Biochemistry. 1996 Feb 20;35(7):2357-64
Authors: Söhndel S, Hu CK, Marti D, Affolter M, Schaller J, Llinás M, Rickli EE
...
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08-22-2010 02:27 PM
[Nature network NMR forum] Journal club: recombinant RNA expression (0 replies)
Journal club: recombinant RNA expression (0 replies)
Recently I was thinking about my RNA project and wishing that I could express RNA in bacteria as is typically done for recombinant proteins. I looked at the July issue of Nature Methods to see a paper describing exactly the technology I was imagining. The authors created plasmids which allow the fusion of a target RNA sequence to a tRNA scaffold which is authenically processed by the cellular machinery and protected from degradation by the intrinsic stability of the tRNA. The potential benefits of this technology for NMR of RNA...
Moving from Recombinant Protein Expression to Biomolecular NMR
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