[KPWU blog] Making Ramachandran plot ?II
Making Ramachandran plot ?II
Long time ago, I made a blog article at here to make a record where I can go back to dig information of Ramachandran plot. But the ranges used in the Ramachandran plot are a bit out of date. The Richardson group has a publication in 2003*(Structure validation by Calpha geometry: phi,psi and Cbeta deviation http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=349&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
nmrlearner
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04-20-2011 02:51 AM
[KPWU blog] [NMRPipe]?making a 3D cube
?making a 3D cube
On Feb 18th, an user asked whether there are ways to make a 3D cube of NMR data at the NMRPipe discussion group at Yahoo. Frank Delaglio, the author of NMRPipe, provided a very very nice script to generate the cube. The script is pasted in this post and user can copy it, save it http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=329&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
nmrlearner
News from NMR blogs
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04-15-2011 01:40 AM
[KPWU blog] PyMOL?making movies
PyMOL?making movies
I found several good online tutorials of making movies using PyMOL and provide the links at here. *I should make some examples by my own next time. PyMOL tips and tricks at Dept. Biochemistry, University of Cambridge How to make a movie PyMOL at Youtube Movie school #6 at PyMOL Wikihttp://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=319&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
Site-specific labeling of nucleotides for making RNA for high resolution NMR studies
Abstract Escherichia coli (E. coli) is a versatile organism for making nucleotides labeled with stable isotopes (13C, 15N, and/or 2H) for structural and molecular dynamics characterizations. Growth of a mutant E. coli strain deficient in the pentose phosphate pathway enzyme glucose-6-phosphate dehydrogenase (K10-1516) on 2-13C-glycerol and 15N-ammonium sulfate in Studier minimal medium enables labeling at sites useful for NMR spectroscopy. However, 13C-sodium formate combined with 13C-2-glycerol in the growth media adds labels to new positions. In the absence of labeled formate, both C5 and...