[NMR images] Side-chain assignment with a 15N-TOCSY-HSQC spectrum
http://www.protein-nmr.org.uk/pictures/assign_theory/assign_15ntocsy_principle.png
8/04/2014 7:46:05 AM GMT
Side-chain assignment with a 15N-TOCSY-HSQC spectrum
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1H-15N HSQC, edited by a 1H inversion recovery and observed in the antiphase component (IR-HSQC-AP)
Could someone explain the experimental theory/basis behind 1H-15N inversion recovery filtered HSQC experiment observed in antiphase (IR-HSQC-AP)? I am still learning basic NMR theory, but would like to know about this particular experiment, which was used to detect paramagnetically broadened backbone resonances. Thanks.
Abstract
Biogenesis of iron–sulfur cluster proteins is a highly regulated process that requires complex protein machineries. In the cytosolic iron–sulfur protein assembly machinery, two human key proteins—NADPH-dependent diflavin oxidoreductase 1 (Ndor1) and...
talderson
NMR Questions and Answers
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09-23-2013 06:01 AM
[Question from NMRWiki Q&A forum] 3d Tocsy-hsqc mgnetization transfer problem
3d Tocsy-hsqc mgnetization transfer problem
Dear All,I acquired a 3d tocsy-hsqc edited on Bruker machine of small protein in micelles with a mixing time of 70ms. The problem is that I do not observe almoust spin system of each aminoacid, in detail I see only cross peaks between NH-HA. I obtained very good results by 3d Noesy-hsqc edited. How can I do to improve the magnetization transfer along the spin system and see all cross peaks? Do you think that this problem is due to the nature?Thank you in advance\
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nmrlearner
News from other NMR forums
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03-06-2012 06:17 PM
[Question from NMRWiki Q&A forum] Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
I am facing problems in calibration of my HCCH-TOCSY spectrum in reference to HBHACONH and HCCCONH spectrum. Can somebody tell me how to solve this issue? Can I autoassign my HCCH-TOCSY spectrum using HBHACONH spectrum
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nmrlearner
News from other NMR forums
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10-02-2011 08:25 AM
[Question from NMRWiki Q&A forum] What's wrong with this spectrum? Bruker TPPI 2D TOCSY
What's wrong with this spectrum? Bruker TPPI 2D TOCSY
Hi,
I think this is probably a basic problem but I'm a bit inexperienced with NMR. I'm just trying to process an ethanol TOCSY spectrum from the biomolecular magnetic resonance data bank, but strange peaks are appearing at the very edge of the data.
The spectrum should look like this (processed in SpinWorks):
<img src="http://qa.nmrwiki.org/upfiles/12889441489353594.png" width="70%"</img>
nmrlearner
News from other NMR forums
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11-05-2010 12:20 PM
[U. of Ottawa NMR Facility Blog] Hsqc - tocsy
HSQC - TOCSY
There are many different NMR techniques used to probe molecular structure. Two very useful methods are the HSQC (or HMQC) sequence used to obtain heteronuclear coupling correlations and the TOCSY sequence used to look at extended homonuclear coupling correlations. These are among the most widely used pulse sequences by chemists to help elucidate the molecular structure of organic molecules. The sequences can also be combined into a single HSQC-TOCSY sequence which is simply an HSQC followed by a TOCSY spin lock. For the case of 1H and 13C in organic molecules, the HSQC-TOCSY...
15N-HSQC-TOCSY spectrum
Linear Mode
