A presentation of some recent work from the Metabolomics and Bioinformatics & Statistics Groups at the Institute of Food Research, Norwich, UK. See the teams' webpages at: www.ifr.ac.uk and www.ifr.ac.uk This video is a shortened version of a presentation given at "Advances in Metabolic Profiling 2011".
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(1)H NMR study of monocrotaline and its metabolites in human blood.
(1)H NMR study of monocrotaline and its metabolites in human blood.
(1)H NMR study of monocrotaline and its metabolites in human blood.
Food Chem Toxicol. 2011 Aug 6;
Authors: Yang YC, Crowder J, Wardle NJ, Yang L, White KN, Wang ZT, Annie Bligh SW
Monocrotaline (MCT) is a naturally occurring hepatotoxic pyrrolizidine alkaloid found in plants. This investigation is aimed at furthering the understanding of the role of blood in mediating the transport of MCT and its reactive metabolites in humans. Reactions of monocrotaline and its metabolites,...
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08-17-2011 01:33 PM
The application of micro-coil NMR probe technology to metabolomics of urine and serum
The application of micro-coil NMR probe technology to metabolomics of urine and serum
Abstract Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the...
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03-09-2011 04:19 AM
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
J Appl Toxicol. 2011 Jan 7;
Authors: Tyagi R, Rana P, Khan AR, Bhatnagar D, Devi MM, Chaturvedi S, Tripathi RP, Khushu S
Thallium (Tl) is a toxic heavy metal and its exposure to the human body causes physiological and biochemical changes due to its interference with potassium-dependent biological reactions. A high-resolution (1) H NMR spectroscopy based metabonomic...
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01-11-2011 11:27 PM
[NMR paper] An NMR study of the interaction between the human copper(I) chaperone and the second
An NMR study of the interaction between the human copper(I) chaperone and the second and fifth metal-binding domains of the Menkes protein.
Related Articles An NMR study of the interaction between the human copper(I) chaperone and the second and fifth metal-binding domains of the Menkes protein.
FEBS J. 2005 Feb;272(3):865-71
Authors: Banci L, Bertini I, Ciofi-Baffoni S, Chasapis CT, Hadjiliadis N, Rosato A
The interaction between the human copper(I) chaperone, HAH1, and one of its two physiological partners, the Menkes disease protein...
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11-24-2010 11:14 PM
[NMR paper] 7Li NMR relaxation study of Li+ binding in human erythrocytes.
7Li NMR relaxation study of Li+ binding in human erythrocytes.
Related Articles 7Li NMR relaxation study of Li+ binding in human erythrocytes.
Biochemistry. 1993 Dec 14;32(49):13490-8
Authors: Rong Q, Espanol M, Mota de Freitas D, Geraldes CF
We used 7Li NMR spin-lattice (T1) and spin-spin (T2) relaxation time measurements to investigate the binding of Li+ in human red blood cell (RBC) suspensions. In RBCs containing 1.4 mM Li+, the intracellular 7Li NMR T2 relaxation value (0.30 +/- 0.03 s) was much smaller than the corresponding T1 value...
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08-22-2010 03:01 AM
[NMR paper] 1H NMR studies of reactions of copper complexes with human blood plasma and urine.
1H NMR studies of reactions of copper complexes with human blood plasma and urine.
Related Articles 1H NMR studies of reactions of copper complexes with human blood plasma and urine.
Biochem Pharmacol. 1992 Jan 22;43(2):137-45
Authors: Bligh SW, Boyle HA, McEwen AB, Sadler PJ, Woodham RH
Reactions of the copper complexes Cu(II)Cl2, 2-, and + (where DIPS is 3,5-diisopropylsalicylate and DMP is 2,9-dimethylphenanthroline) with human blood plasma and urine have been studied by 500 MHz 1H NMR spectroscopy, and CD spectroscopy has been used to...
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08-21-2010 11:41 PM
[NMR paper] 1H and 15N NMR study of human lysozyme.
1H and 15N NMR study of human lysozyme.
Related Articles 1H and 15N NMR study of human lysozyme.
J Biochem. 1991 Dec;110(6):1022-9
Authors: Ohkubo T, Taniyama Y, Kikuchi M
The 15N signal assignment of human lysozyme was carried out by using 1H-1H and 1H-15N two dimensional experiments. To solve the severe overlap problem of the NH signals, uniform labeling of the protein with 15N was introduced. The uniformly 15N labeled protein was prepared using a high-expression system of Saccharomyces cerevisiae. From the analyses of 1H and 15N NMR...
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08-21-2010 11:12 PM
[NMR paper] 1H and 15N NMR study of human lysozyme.
1H and 15N NMR study of human lysozyme.
Related Articles 1H and 15N NMR study of human lysozyme.
J Biochem. 1991 Dec;110(6):1022-9
Authors: Ohkubo T, Taniyama Y, Kikuchi M
The 15N signal assignment of human lysozyme was carried out by using 1H-1H and 1H-15N two dimensional experiments. To solve the severe overlap problem of the NH signals, uniform labeling of the protein with 15N was introduced. The uniformly 15N labeled protein was prepared using a high-expression system of Saccharomyces cerevisiae. From the analyses of 1H and 15N NMR...
1H NMR Metabolomics Study of Human Urine
Linear Mode
